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背景与目的:在肿瘤的发生、发展和血管形成中,环氧合酶-2(cyc looxygenase-2,COX-2)起了很重要的作用,而多种COX-2抑制剂可以抑制肿瘤细胞增殖,诱导肿瘤细胞凋亡。本实验研究COX-2抑制剂NS398抑制人骨肉瘤细胞MG-63增殖及诱导凋亡的效果。方法:MTT比色观察NS398的细胞毒性作用及其浓度依赖性;Hoechst 33258荧光染色、透射电子显微镜和TUNEL观察细胞凋亡的形态学变化;流式细胞仪检测细胞凋亡率及其时间依赖性。结果:分别以1、10、50、100、200μmol/L NS398作用后,细胞生长受到不同程度抑制(14.7%,23.5%,33.6%,52.5%和81.4%);荧光显微镜、电镜和TUNEL观察到细胞胞质浓缩、核凝聚、核碎裂和凋亡小体形成。流式细胞议显示NS398 100μmol/l作用24h、48h、72h后,细胞凋亡率分别与对照组比较有非常显著性差异(P<0.01)。结论:NS398能抑制人MG-63细胞增殖,诱导细胞凋亡。
BACKGROUND & OBJECTIVE: Cyclooxygenase-2 (COX-2) plays an important role in tumorigenesis, development and angiogenesis. A variety of COX-2 inhibitors can inhibit tumor cells Proliferation, induce tumor cell apoptosis. This experiment study COX-2 inhibitor NS398 inhibition of human osteosarcoma MG-63 proliferation and induction of apoptosis. Methods: The cytotoxicity and concentration dependence of NS398 were observed by MTT colorimetric assay. Morphological changes of apoptosis were observed by Hoechst 33258 staining, transmission electron microscopy and TUNEL staining. Flow cytometry was used to detect the apoptosis rate and the time-dependent manner . RESULTS: After treated with 1, 10, 50, 100 and 200μmol / L NS398 respectively, cell growth was inhibited to a certain extent (14.7%, 23.5%, 33.6%, 52.5% and 81.4%). Fluorescence microscopy, electron microscopy and TUNEL Cell cytoplasm concentration, nuclear condensation, nuclear fragmentation and apoptotic body formation. Flow cytometry showed that the apoptotic rate of NS398 cells treated with 100μmol / l of NS398 for 24h, 48h and 72h were significantly different from that of the control group (P <0.01). Conclusion: NS398 can inhibit the proliferation of human MG-63 cells and induce apoptosis.