论文部分内容阅读
研究了在PBS缓冲介质中,一种检测癌胚抗原的新型免标记电化学免疫传感器的制备及应用,石墨烯与甲苯胺蓝复合物饰于玻碳电极表面,通过循环伏安法对修饰的电极进行表征。基于以[Fe(CN)6]3-/4-为氧化还原探针,癌胚抗原抗体反应引起[Fe(CN)6]3-/4-探针的电流响应的变化,来实现癌胚抗原的检测,癌胚抗原的浓度在0.3～10.0 ng/mL范围内与峰电流呈良好的线性关系,回归方程为Δi=-1.926ρ+0.413,相关系数为0.9903,检测限为0.1 ng/mL,该传感器具有良好的重现性、选择性和稳定性,用于人血清样品的测定获得满意结果。
The preparation and application of a novel label-free electrochemical immunosensor for the detection of carcinoembryonic antigen in PBS buffer medium were studied. The graphene and toluidine blue complex were decorated on the surface of glassy carbon electrode. The modified Electrode characterization. Based on the change of the current response of [Fe (CN) 6] 3- / 4-probe by [Fe (CN) 6] 3- / 4- as the redox probe and the carcinoembryonic antigen-antibody reaction, Antigen detection and CEA showed a good linear relationship with peak current in the range of 0.3 ~ 10.0 ng / mL. The regression equation was Δi = -1.926ρ + 0.413, with a correlation coefficient of 0.9903 and a detection limit of 0.1 ng / mL , The sensor has good reproducibility, selectivity and stability for the determination of human serum samples obtained satisfactory results.