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为有效防治烟草黑胫病,筛选了3株可高效拮抗烟草黑胫病菌(Phytophthora parasitica var.nicotianae)的生防菌ZH、C9和C20,经生理生化和基因序列分析,3株菌都被鉴定为解淀粉芽胞杆菌(Bacillus amyloliquefaciens).以ZH为代表性菌株进行定殖实验.结果显示,解淀粉芽胞杆菌ZH在土壤和烟草植株体内都具有良好的定殖能力,123 d后根际土壤和根围土壤中生防菌的生物量分别达到9.07×106 CFU/g和1.2×107 CFU/g,烟草茎、叶内的生防菌生物量分别达到1.83×104CFU/g和5.58×104 CFU/g.以正常施用化学药剂的烟草为对照,以ZH、C9和C20混合菌剂进行生防菌大田应用试验.与对照相比,施用混合菌株发酵液不同时期的防效为85.37%-95.11%.同时,烟草的产量相比对照每公顷增产20.18%,每公顷增值31.27%.本研究提供了一种新型高效的烟草黑胫病生防菌及其大田应用方法,具有重要的应用价值.图3表4参27
In order to effectively control tobacco black shank, three strains of biocontrol bacteria ZH, C9 and C20 that can effectively antagonize Phytophthora parasitica var. Nicotianae were screened, and three strains were identified by physiological and biochemical analysis and gene sequence analysis Was Bacillus amyloliquefaciens.The colonization experiment with ZH as a representative strain showed that Bacillus amyloliquefaciens ZH had good colonization ability in soil and tobacco plants and that 123 days after rhizosphere soil and The biomass of biocontrol bacteria in rhizosphere soil reached 9.07 × 106 CFU / g and 1.2 × 107 CFU / g, respectively. The biocontrol bacteria biomass in stem and leaf of tobacco reached 1.83 × 104 CFU / g and 5.58 × 104 CFU / g.Compared with the control, the control effect of mixed strains of fermentation broth at different periods was 85.37% -95.11% At the same time, the yield of tobacco increased 20.18% per hectare compared with the control, which increased 31.27% per hectare.This study provided a new and efficient biocontrol bacterium for tobacco black shank and its field application method, which has important application value. 3 Table 4 Reference 27