论文部分内容阅读
洋桔梗为龙胆科观赏植物,原产于北美洲,在欧洲、日本及美国的销售量急速上升,但在我国还处于引种试验阶段,没有形成规模种植,种苗市场需求量很大。但其种子细小,价格较贵,从发芽到正常生长有3～4个月的停滞期,这段时期内,往往有大量的幼苗死亡,发芽率很低,育苗困难,扦插繁殖也很困难。若组培快繁,不仅可在短期内大量繁殖,加速推广应用,还可对其进行种质保存。材料消毒洋桔梗种子非常细小,1毫升种子约有1.7万粒,消毒和接种操作需非常细致。取种子数枚,在超净工作台(预先工作15分钟以上)上用0.1%HgC12水溶液浸泡5分钟,小心倾去 HgC12水溶液,防止种子跟着流出来,在留有少量 HgC12溶液的情况下用接种刀将种子转移到无菌水中冲洗5次,备用。培养条件种子萌发培养基(1)MS+6-BA 0.5+NAA 0.05+GA 0.5;继代培养基(2)MS+6-BA 0.5+NAA 0.02;生根培养基(3)1/2MS+NAA 0.8。培养基加琼脂0.7%,(1)、(2)加蔗糖3%,(3)加蔗糖2%,pH 值调至5.8。培养温度25+2℃,光照
Eustoma is a gentian ornamental plant, native to North America. Its sales in Europe, Japan and the United States have risen sharply. However, it is still in the stage of introduction test in our country. There is no scale planting and there is a great demand for the seed market. However, its seeds are small and expensive, with a stagnant period of 3 to 4 months from germination to normal growth. During this period, a large number of seedlings often die, the germination rate is very low, breeding is difficult, and cutting propagation is also very difficult. If the tissue culture fast propagation, not only in the short term large-scale breeding, accelerate the promotion and application, but also its germplasm preservation. Material disinfection Eustoma seeds are very small, about 1 ml seed of about 17000, disinfection and vaccination operations need to be very meticulous. Take a few pieces of seeds and soak in 0.1% HgC12 aqueous solution on a clean bench (15 minutes in advance) for 5 minutes. Carefully pour off the HgC12 solution to prevent the seeds from flowing out. With a small amount of HgC12 solution, Transfer seed to sterile water for 5 times and set aside. (1) MS + 6-BA 0.5 + NAA 0.05 + GA 0.5; subculture medium (2) MS + 6-BA 0.5 + NAA 0.02; rooting medium (3) 1/2 MS + NAA 0.8. Medium plus agar 0.7%, (1), (2) plus sucrose 3%, (3) add 2% sucrose, the pH adjusted to 5.8. Culture temperature 25 +2 ℃, light