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目的:探讨转化生长因子β1(TGF-β1)和碱性成纤维生长因子(b FGF)与儿童先天性胆管扩张症(CBD)的关系。方法:采用免疫组化方法检测85例CBD患儿病理标本中TGF-β1和b FGF蛋白表达。结果:TGF-β1和b FGF蛋白在不同胆管扩张症患儿的胆管壁、胆囊壁均有不同程度的表达,两者在胆管壁的表达大于胆囊壁,且不同分型、亚型间的表达程度有明显差异,但均高于正常胆管壁与胆囊壁,其中II型中两者表达最强(均P<0.05);TGF-β1与b FGF蛋白CBD胆管壁与胆囊壁中的表达随黏膜细胞脱落程度、增生肥厚程度,炎症细胞浸润程度加重而升高(均P<0.05);TGF-β1与b FGF蛋白在CBD胆管壁与胆囊壁中的表达均呈明显正相关(r=0.893;r=0.953,均P<0.05)。结论:TGF-β1与b FGF可能在CDB的发生发展中起了重要的协同作用,有望为从分子生物学角度治疗CBD提供新的途径。
Objective: To investigate the relationship between transforming growth factor β1 (TGF-β1) and basic fibroblast growth factor (b FGF) in children with congenital bile duct dilatation (CBD). Methods: Immunohistochemistry was used to detect the expression of TGF-β1 and b FGF in pathological specimens of 85 children with CBD. Results: The expression of TGF-β1 and b FGF protein in bile duct wall and gallbladder wall were different in children with different biliary dilatation. The expression of TGF-β1 and b FGF in gallbladder wall was higher than that in gallbladder wall, and the expression of TGF- (P <0.05). The expression of TGF-β1 and b FGF protein in the bile ducts and gallbladder wall of the bile duct with mucosa (P0.05). The expression of TGF-β1 and b FGF protein in the bile duct wall and gallbladder wall were positively correlated with each other (r = 0.893; r = 0.953, all P <0.05). CONCLUSION: TGF-β1 and b FGF may play an important synergistic role in the development of CDB. It is expected to provide a new approach for the treatment of CBD from molecular biology perspective.