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目的：研究日本血吸虫重组谷胱甘肽－Ｓ－转移酶（ｒｅＳｊｃ２６ＧＳＴ）在不同品系小鼠（ＢＡＬＢ／ｃ小鼠及Ｃ５７ＢＬ／６小鼠）诱生免疫应答的差异。方法：将ｒｅＳｊｃ２６ＧＳＴ（弗氏佐剂）经皮下免疫ＢＡＬＢ／ｃ小鼠及Ｃ５７ＢＬ／６小鼠，收集免疫前和免疫后血清及取小鼠脾脏，分析ｒｅＳｊｃ２６ＧＳＴ免疫小鼠诱生的特异性抗体、脾脏淋巴细胞特异性增殖能力及细胞因子种类等。结果：ｒｅＳｊｃ２６ＧＳＴ免疫Ｃ５７ＢＬ／６小鼠所诱生的抗体总ＩｇＧ及ＩｇＧ亚类均较明显高于免疫ＢＡＬＢ／ｃ小鼠。抗体ＩｇＧ亚类分析显示，ｒｅＳｊｃ２６ＧＳＴ在Ｃ５７ＢＬ／６小鼠诱生较高滴度的ＩｇＧ１和ＩｇＧ２ａ及ＩｇＧ２ｂ（血清按１∶４００稀释，Ａ４９２ｎｍ值分别为＞３．０、１．１２７、＞３．０），而在ＢＡＬＢ／ｃ小鼠主要诱生较低滴度的ＩｇＧ１和ＩｇＧ２ａ及ＩｇＧ２ｂ（血清按１∶４００稀释，Ａ４９２ｎｍ值分别为１．１８９、０．３９、０．６２５）；ｒｅＳｊｃ２６ＧＳＴ免疫能够诱导小鼠Ｔｈ细胞激活。细胞因子分析显示ｒｅＳｊｃ２６ＧＳＴ免疫Ｃ５７ＢＬ／６小鼠既能诱生较高水平的ＩＬ－２及ＩＦＮ－γ，又能诱生ＩＬ－５。而ｒｅＳｊｃ２６ＧＳＴ免疫ＢＡＬＢ／ｃ小鼠仅?
OBJECTIVE: To study the differences in the immune responses induced by recombinant human recombinant Schistosoma japonicum Schistosoma japonicum Schistosoma japonicum SR-26GST in BALB / c mice and C57BL / 6 mice. Method: The reSjc26GST (Freund’s adjuvant) subcutaneously immunized BALB / c mice and C57BL / 6 mice were collected before immunization and serum taken after immunization and spleens of mice, analysis of specific antibodies induced in mice immunized reSjc26GST, Spleen lymphocyte-specific proliferation and cytokine types. Results: The total IgG and IgG subclasses induced by reSjc26GST immunized C57BL / 6 mice were significantly higher than that of immunized BALB / c mice. IgG subclass antibody analysis, reSjc26GST (serum diluted in C57BL / 6 mice induced higher titers of IgG1 and IgG2a and IgG2b by 1:400, A492nm values were> 3.0,1.127,> 3. 0), whereas in BALB / c mice, lower titers of IgG1 and IgG2a and IgG2b were predominantly induced (serum dilutions at 1: 400, A492nm values were 1.189, 0.39, 0.625 respectively); reSjc26GST immunization Can induce mouse Th cell activation. Cytokine analysis showed that reSjc26GST immunized C57BL / 6 mice induced both high levels of IL-2 and IFN-γ as well as IL-5. While reSjc26GST immunized BALB / c mice only?