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目的研究丙烯酰胺(ACR)对钙调蛋白(CaM)和蛋白激酶C(PKC)含量的影响。方法将27只健康SPF级成年雄性Wistar大鼠随机分为3组,分别为对照组(双蒸水)和20、40mg/kgACR染毒组,每组9只。采用腹腔注射方式染毒,隔天染毒,连续染毒8周。每周测量大鼠体重和步态得分。染毒结束后,迅速分离脊髓组织,采用ELISA试剂盒测定CaM的含量,采用聚丙烯酰胺凝胶电泳(SDS-PAGE)和免疫印迹法(WesternBlotting)测定蛋白激酶C(PKC)的含量。结果对照组大鼠的步态得分均为1分;20、40mg/kgACR染毒组分别自第7、3周开始出现步态得分增高,差异均有统计学意义(P<0.05或P<0.01)。与对照组相比,20、40mg/kgACR染毒组大鼠脊髓组织CaM含量均下降(P<0.01),40mg/kgACR染毒组大鼠脊髓组织PKC含量下降(P<0.05),差异有统计学意义。结论 ACR可降低大鼠脊髓组织CaM和PKC的含量,造成对神经丝(NF)的损害,这可能是ACR所致神经性疾病的发病机制之一。
Objective To investigate the effect of acrylamide on the content of calmodulin (CaM) and protein kinase C (PKC). Methods Twenty-seven adult male Sprague-Dawley (Wistar) male Wistar rats were randomly divided into three groups: control group (double distilled water) and 20,40 mg / kg ACR group, with 9 rats in each group. The way of intraperitoneal injection, the next day exposure, continuous exposure for 8 weeks. Weights and gait scores were measured weekly. Spinal cord tissue was quickly isolated after exposure to the disease. The content of CaM was measured by ELISA kit. Protein kinase C (PKC) content was determined by SDS-PAGE and Western Blotting. Results The gait scores of the control group were all 1 point. Gait scores increased in the 20 and 40 mg / kg ACR groups from the 7th and the 3rd week, respectively, and the difference was statistically significant (P <0.05 or P <0.01 ). Compared with the control group, the content of CaM in the spinal cord of 20,40 mg / kg ACR group decreased (P <0.01), and the PKC content in the spinal cord of 40 mg / kg ACR group decreased (P <0.05) Significance of learning. Conclusion ACR can reduce the content of CaM and PKC in the spinal cord of rats and cause damage to nerve filaments (NF), which may be one of the pathogenesis of neurological diseases caused by ACR.