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本研究应用体外培养的人胚肺成纤维细胞(2BS)和人宫颈癌细胞(Hela)为实验模型。在培养液中分别加入人参根总皂甙(SRG)和人参皂甙Rb_1、Rg_1、Re、Rh_1孵育3～7d后,用MTT法和细胞计数法测定细胞的增殖。MTT测定结果表明,Rb_1、Rg_1、Re、Rh_1可以促进衰老细胞的增殖,但对未衰老的细胞增殖没有显著影响,对Hela细胞的增殖有抑制作用。细胞计数法测定结果表明,总皂甙和4种皂甙单体都可以降低Hela细胞的群体增殖率和克隆生长率,其中Re、Rh_1作用显著。同时,增加衰老细胞的群体增殖率和克隆生长率,其中Rb_1和Rg_1作用显著。
This study used in vitro cultured human embryonic lung fibroblasts (2BS) and human cervical cancer cells (Hela) as experimental models. After the cells were incubated with ginsenoside total root saponins (SRG) and ginsenosides Rb_1, Rg_1, Re, and Rh_1, respectively, for 3 to 7 days, cell proliferation was measured by MTT method and cell counting method. MTT assay results showed that Rb_1, Rg_1, Re, and Rh_1 could promote the proliferation of senescent cells, but had no significant effect on the proliferation of non-senescent cells, and inhibited the proliferation of Hela cells. The results of cell counting assay showed that the total saponins and four saponin monomers could reduce the population proliferation rate and clone growth rate of Hela cells, of which Re, Rh_1 had a significant effect. At the same time, population proliferation rate and clonal growth rate of senescent cells were increased, among which Rb_1 and Rg_1 had significant effects.