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目的:比较髓系白血病细胞株NB4细胞和HL60细胞向粒细胞分化过程中细胞表面抗原CD11b和CD18的表达。方法:用全反式维甲酸(ATRA)诱导NB4细胞和HL60细胞向粒细胞定向分化,用MGG染色法进行初步鉴定,用FACS检测并比较两株细胞在分化前后细胞表面CD11b和CD18的变化,用Western blot检测两株细胞分化前后CD18的蛋白表达。结果:与分化前相比,两株细胞分化后细胞表面CD11b和CD18的表达均明显升高,并且这样的变化在NB4细胞中较HL60细胞更加显著。结论:分化NB4细胞可能较分化HL60细胞是更方便的粒细胞模型。
OBJECTIVE: To compare the expression of cell surface antigen CD11b and CD18 during the differentiation of myeloid leukemia NB4 cells and HL60 cells into granulocytes. Methods: ATRA was used to induce the differentiation of NB4 cells and HL60 cells into granulocytes. The expression of CD11b and CD18 was detected by FACS before and after differentiation. Western blot was used to detect the protein expression of CD18 before and after differentiation. Results: Compared with pre-differentiation, the expression of CD11b and CD18 on the cell surface were significantly increased after differentiation, and such changes were more significant in NB4 cells than HL60 cells. Conclusion: Differentiation of NB4 cells may be more convenient than differentiation of HL60 cells in granulocyte models.