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本实验研究不同强度静电磁场对体外培养大鼠骨髓间充质干细胞增殖与分化作用.体外分离培养大鼠骨髓间充质干细胞,传代后随机分为6组,分别用强度为0(对照组)、0.9、1.2、1.5、1.8和2.1 mT的静电磁场处理,每d每次处理30 min.在磁场处理后的9～10 d,骨髓间充质干细胞开始出现钙化小颗粒.0.9 mT组抑制骨髓间充质干细胞增殖,1.5到2.1mT组促进骨髓间充质干细胞增殖.在磁场处理后的12 d和15 d,1.5和1.8 mT组极显著地增加了碱性磷酸酶(AKP)活性.采用AKP组织化学染色和钙化结节染色对骨髓间充质干细胞成骨性分化进行鉴定,AKP组织化学染色和钙化结节染色都呈现了极强的阳性结果,尤以1.5 mT和1.8 mT阳性染色面积最大.在SEMFs处理后的48 h和96 h,1.5 mT和1.8 mT组胶原I(collagen-Ⅰ)和骨形态发生蛋白2(bonemorphogenetic protein-2,Bmp-2)基因表达水平显著高于对照组.在SEMFs处理后的12 d,BMP-2蛋白表达量高于对照组.研究表明,0.9 mT组抑制骨髓间充质干细胞增殖,1.5 mT到2.1 mT组不同强度静电磁场促进体外培养骨髓间充质干细胞的增殖.磁场组能促进骨髓间充质干细胞成骨性分化,其中尤以1.5 mT和1.8 mT组促进大鼠骨髓间充质干细胞分化作用效果最为明显.
In this study, the effects of different electrostatic fields on the proliferation and differentiation of rat bone marrow mesenchymal stem cells cultured in vitro were studied.Malone bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured in vitro and were randomly divided into 6 groups: control group, , 0.9, 1.2, 1.5, 1.8 and 2.1 mT static magnetic field treatment, each d treatment for 30 min.After magnetic field treatment 9 ~ 10 d, bone marrow mesenchymal stem cells began to appear calcified small particles .0.9 mT group inhibited bone marrow Mesenchymal stem cell proliferation, 1.5 to 2.1mT group to promote bone marrow mesenchymal stem cell proliferation in the magnetic field after 12 d and 15 d, 1.5 and 1.8 mT group significantly increased alkaline phosphatase (AKP) activity using AKP histochemical staining and calcified nodules staining bone marrow mesenchymal stem cells osteogenic differentiation were identified, AKP histochemical staining and calcified nodules showed strong positive results, especially 1.5 mT and 1.8 mT positive staining area At 48 and 96 h after SEMFs treatment, the mRNA expression of collagen-Ⅰ and Bmp-2 in 1.5 mT and 1.8 mT groups was significantly higher than that in the control group At 12 days after SEMFs treatment, the BMP-2 protein table The results showed that 0.9 mT group inhibited the proliferation of BMSCs and 1.5 mT to 2.1 mT groups with different intensities of electrostatic field to promote the proliferation of BMSCs in vitro.Field group could promote the proliferation of BMSCs Osteogenic differentiation, especially in 1.5 mT and 1.8 mT group to promote the differentiation of rat bone marrow mesenchymal stem cells the most obvious effect.