论文部分内容阅读
Objective: To investigate the apoptosis-inducing effect of Jinke on Molt-4 cells and its possible mechanism.Methods: The Molt-4 cells were treated with different concentrations of Jinke and then cultured for necessary time.The Annexin-V / PI method was used to detect the apoptosis rate.The cell cycle was analyzed by DNA content with flow cytometry.Double parameters analysis of cyclins/ DNA was performed to detect the expression of cyclin E.API method was used to confirm the cell cycle-specific apoptosis.The expressions of Bcl-2 and Bax were detected by western blot.Results: 24 h after the treatment of 0.5, 1.0, 1.5, 2.0 and 3.0 mg/mL Jinke, the apoptosis rate of Molt-4 cells was evaluated in a concentration-dependent manner, from 5.2% of the control group to 41.0% of the 3.0 mg/mL Jinke group.When the Molt-4 cells were cultured with 1.5 mg/mL Jinke, the apoptosis rate was evaluated in a time-dependent manner.DNA content analysis showed that G0/G1 phase of Molt-4 cells increased in a time-dependent manner.The expression of cyclin E increased gradually.API assay showed the apoptosis cells were almost in G0/G1 phase.Western blot showed the Bcl-2 was down-regulated and the Bax was up-regulated.Conclusion: Jinke could induce G1 phase-specific apoptosis in Molt-4 cells in time- and concentration-dependent manners involving G1 phase arrest.The mechanism of apoptosis inducing effect may be related to the upregulation of Bax and the down-regulation of Bci-2.