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雄性Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ大鼠皮下埋置１７－β雌二醇（１７－β－ｅｓｔｒａｄｉｏｌ，Ｅ２）药泵诱发垂体催乳素（ｐｒｏｌａｃｔｉｎ，ＰＲＬ）瘤，并每日皮下注射褪黑素（ｍｅｌａｔｏｎｉｎ，ＭＬＴ），观察ＭＬＴ对Ｅ２诱发ＰＲＬ瘤生长的影响。另外，采用放免法和紫外分光光度法测定大鼠血浆ＰＲＬ和过氧化脂质（ｐｅｒｏｘｉｄａｔｉｖｅ ｌｉｐｉｄ，ＰＬ）浓度，观察ＰＲＬ瘤重量与大鼠血浆 ＰＲＬ、ＰＬ浓度间的相关关系。实验结果显示，在对照组、０．０５、０．２５、０．５０、１．００和 ２．００ｍｇ ＭＬＴ组，ＰＲＬ瘤重量分别为１１５．０±７１．０、８５．２±４１．０、５８．９±２４．１、７２．７±２３．６、７９．３±５６．１、７４．５±４６．８ｍｇ；血浆ＰＲＬ浓度分别为４９３．４６±３３．３、３７３．７８±２６．５、１２５．１３±１３．３、２０１．７９±１１．２、４１８．８８±４１．３、２８１．９４±３６．４ｎｇ／ｍｌ；血浆ＰＬ水平分别为１．２１±０．２３、０．８９±０．３２、０．９２±０．２７、０．６４±０．２４、０．４１±０．１４、０．４３±０．２１△Ｄ２３３／ｍｌ。相关性分析表明，ＰＲＬ瘤重量与血浆 ＰＲＬ浓度间的相?
Male Sprague-Dawley rats were implanted with 17-β-estradiol (E2) pump to induce pituitary prolactin (PRL) tumors. Subcutaneous injection of melatonin (MLT) was performed daily. To observe the effect of MLT on E2-induced growth of PRL tumors. In addition, plasma PRL and peroxidative lipid (PL) concentrations in rats were determined by radioimmunoassay and ultraviolet spectrophotometry. The correlation between PRL tumor weight and plasma PRL and PL concentrations in rats was observed. The experimental results showed that in the control group, 0.05, 0.25, 0.50, 1.00, and 2.00 mg MLT groups, the PRL tumor weights were 115.0±71.0, 85.2±41.0, respectively. 58.9±24.1, 72.7±23.6, 79.3±56.1, 74.5±46.8 mg; plasma PRL concentrations were 493.46±33.3 and 373.78±, respectively. 26.5, 125.13±13.3, 201.79±11.2, 418.88±41.3, 281.94±36.4 ng/ml; plasma PL levels were 1.21±0.23, respectively 0.89±0.32, 0.92±0.27, 0.64±0.24, 0.41±0.14, 0.43±0.21 △D233/ml. Correlation analysis showed that the phase between PRL tumor weight and plasma PRL concentration was comparable.