论文部分内容阅读
目的研究S型氯代甘油醇(SACH)对大鼠精子运动和超活化的影响和可能机制。方法 20只成熟雄性SD大鼠随机分为4组,分别给予0、2.5、5.0和10mg/kg BW SACH,连续灌胃染毒52天。取附睾尾精子,获能条件下培养5h,以计算机辅助精子分析系统检测精子运动和超活化运动,同时检测精子特异的3-磷酸甘油醛脱氢酶(GAPDS)活性、三磷酸腺苷(ATP)和环磷酸腺苷(cAMP)水平,观察己酮可可碱(PTF)对SACH的拮抗作用。结果 SACH染毒组大鼠精子曲线运动速率(VCL)、平均路径速率(VAP)、直线运动速率(VSL)和精子头侧摆幅度(ALH)与对照组相比受到显著抑制(P<0.01),运动直线性(LIN)显著增强(P<0.01)。SACH使VCL≥400μm/s或LIN≤20%的比例显著减少(P<0.01和P<0.05),表明精子超活化受抑制。SACH显著抑制GAPDS活性,ATP和cAMP水平也随染毒剂量增加呈下降趋势。PTF可改善精子cAMP水平,部分缓解精子运动和超活化的抑制程度。结论 SACH抑制大鼠精子GAPDS活性导致ATP和cAMP水平下降,可能是影响精子运动和超活化的重要机制。
Objective To investigate the effects and possible mechanisms of S-chloro-glycerol (SACH) on sperm motility and hyperactivation in rats. Methods Twenty male Sprague-Dawley rats were randomly divided into 4 groups, and received 0, 2.5, 5.0 and 10 mg / kg BW SACH, respectively. The animals were continuously gavaged for 52 days. Sperm motility and hyperactivation were detected by computer-aided sperm analysis system. Sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) activity, adenosine triphosphate (ATP) The level of cAMP was measured to observe the antagonism of pentoxifylline (PTF) to SACH. Results Compared with control group, the sperm velocity (VCL), mean path velocity (VAP), linear velocity (VSL) and sperm head swing amplitude (ALH) in SACH group were significantly inhibited (P <0.01) , Motor linearity (LIN) increased significantly (P <0.01). SACH significantly reduced the proportion of VCL ≥400μm / s or LIN≤20% (P <0.01 and P <0.05), indicating that the activation of spermatozoa was inhibited. SACH significantly inhibited the activity of GAPDS, ATP and cAMP levels also decreased with the dose increased. PTF can improve sperm cAMP levels, partially alleviate the degree of inhibition of sperm motility and hyperactivation. Conclusion SACH can inhibit the activity of GAPDS in spermatozoa and lead to the decrease of ATP and cAMP, which may be one of the important mechanisms that affect sperm motility and hyperactivation.