论文部分内容阅读
目的：研究三尖杉酯碱（Ｈａｒ）对膜脂过氧化及膜脂运动的影响．方法：膜脂过氧化用硫代巴比妥酸法测定，其含量用ＭＡＡ表示；用纳秒荧光偏振技术研究膜脂过氧化对膜脂分子运动的影响．结果：Ｈａｒ１０ｍｇ·Ｌ－１可使ＭＡＡ由１７±０８增加至５４±５１μｍｏｌ／ｇ蛋白质；随过氧化脂质含量的增加，膜脂双层的微粘度增加，磷脂分子摆动角减小（ｒ＝－０８５６５，Ｐ＜００１），Ｃａ２＋转运ＡＴＰ酶活性降低（ｒ＝－０８７１４，Ｐ＜００１）；ＤＰＨ的荧光强度减弱，荧光寿命缩短．结论：Ｈａｒ引起肌浆网膜脂过氧化，因而影响膜脂双层的运动和膜蛋白的功能．
Objective: To study the effect of Harrington on membrane lipid peroxidation and membrane lipid metabolism. Methods: Membrane lipid peroxidation was measured by thiobarbituric acid method and its content was expressed by MAA. The influence of membrane lipid peroxidation on the molecular motion of membrane lipid was studied by nanosecond fluorescence polarization technique. Results: HarA10mg · L-1 could increase the MAA from 1.7 ± 0.5 to 5.4 ± 5.11μmol / g protein. With the increase of lipid peroxidation, the microviscosity of membrane lipid bilayer increased. (R = -08565, P <001), the activity of Ca2 + transport ATPase decreased (r = -08714, P <001); the fluorescence intensity of DPH weakened and the fluorescence lifetime shortened . Conclusion: Har caused sarcoplasmic reticulum lipid peroxidation and thus affected membrane lipid bilayer motility and membrane protein function.