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目的探讨乳腺癌组织中微血管生成及nm23表达的关系及其临床意义。方法应用SP免疫组织化学染色方法 ,以CD3 4单克隆抗体标记6 5例乳腺癌肿瘤组织中微血管,计数微血管密度(MVD),同时检测肿瘤组织中nm23的表达,分析乳腺癌组织血管生成和nm23的表达及两者与肿瘤生物学行为的关系。结果全部肿瘤标本有不同程度的微血管生成,并表现出明显的异质性。MVD计数为3～33个/400倍视野(平均11.6±7.0),nm23阳性表达率为81.5%。MVD与nm23的表达呈负相关(P=0.011),且两者分别与患者年龄、肿瘤大小无明显关系,而与肿瘤淋巴转移有关(P=0.0 1 4,0.0 0 5)。nm2 3的表达与肿瘤临床分期有关(P=0.0 3 8)。结论乳腺癌肿瘤组织中的微血管生成促进了淋巴转移,而nm23的表达可抑制淋巴转移;联合检测MVD及nm23对评估肿瘤淋巴转移风险具有一定的临床意义,可预测患者的预后。
Objective To investigate the relationship between microvascular angiogenesis and nm23 expression in breast cancer and its clinical significance. Methods The SP immunohistochemical staining method was used to mark the microvessels in 65 cases of breast cancer tissues with CD3 4 monoclonal antibody and to count the microvessel density (MVD). At the same time, the expression of nm23 in the tumor tissue was detected. The angiogenesis and nm23 The relationship between the two and tumor biological behavior. Results All tumor samples had different degrees of microvascular angiogenesis and showed obvious heterogeneity. The MVD counts ranged from 3 to 33/400 times (average 11.6 ± 7.0), and the nm23 positive rate was 81.5%. There was a negative correlation between MVD and nm23 expression (P = 0.011). There was no significant correlation between MVD and tumor size (P = 0.014, P = 0.011). The expression of nm23 was related to the clinical stage (P = 0.038). Conclusions MVD in breast cancer tissue can promote lymphatic metastasis, while the expression of nm23 can inhibit lymphatic metastasis. Combined detection of MVD and nm23 has some clinical significance in assessing the risk of lymphatic metastasis, and can predict the prognosis of patients.