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目的:观察PKM2和HIF-1α在人喉鳞状细胞癌中的表达,分析其在喉鳞状细胞癌发生、发展中的作用和关系。方法:37例喉癌组织及癌旁组织取自2013-06-2014-06期间河北医科大学第二医院住院手术的喉癌患者,采用SP免疫组织化学方法检测喉鳞状细胞癌组织及癌旁正常组织中PKM2和HIF-1α的表达情况,所有实验数据用SPSS 13.0统计软件分析。结果:PKM2及HIF-1α在癌组织中的阳性表达率分别为62.16%和64.86%,在癌旁组织中的阳性表达率为13.51%、21.62%,差异有统计学意义(P<0.01)。PKM2及HIF-1α在高分化喉癌组织中表达的阳性率均为47.83%,在中~低分化癌组织中表达的阳性率分别为85.71%和92.86%,均差异有统计学意义(P<0.05)。PKM2及HIF-1α在淋巴结转移喉癌组织中的阳性表达率分别为90.00%和100.00%,在无淋巴结转移喉癌组织中的阳性表达率均为51.85%,均差异有统计学意义(P<0.05)。HIF-1α在Ⅰ~Ⅱ期、Ⅲ~Ⅳ期喉癌中的阳性表达率为53.85%、90.91%,组间比较差异有统计学意义(P<0.05)。PKM2和HIF-1α蛋白的表达与喉癌患者年龄及吸烟量无关(P>0.05)。PKM2与HIF-1α在喉癌细胞中的表达具有相关性(r=0.476,P<0.01)。结论:PKM2及HIF-1α与喉癌细胞的增殖、侵袭及转移关系密切,其蛋白检测可作为生物学指标,为喉癌的诊断与筛查提供了一定的理论基础。
OBJECTIVE: To observe the expression of PKM2 and HIF-1α in human laryngeal squamous cell carcinoma and to analyze the role and relationship between PKM2 and HIF-1α in the occurrence and development of laryngeal squamous cell carcinoma. Methods: Thirty-seven laryngeal cancer tissues and para-cancerous tissues were obtained from laryngeal cancer patients hospitalized in the Second Hospital of Hebei Medical University during 2013-06-2014-06. SP immunohistochemical method was used to detect the expression of laryngeal squamous cell carcinoma The normal tissues of PKM2 and HIF-1α expression, all experimental data using SPSS 13.0 statistical software analysis. Results: The positive rates of PKM2 and HIF-1α in cancer tissue were 62.16% and 64.86%, respectively. The positive rates of PKM2 and HIF-1α in cancer tissues were 13.51% and 21.62%, respectively, with statistical significance (P <0.01). The positive rates of PKM2 and HIF-1α expression in well-differentiated laryngeal squamous cell carcinoma were 47.83%, respectively. The positive rates of PKM2 and HIF-1α in moderate-poorly differentiated carcinoma tissues were 85.71% and 92.86%, respectively, with statistical significance (P < 0.05). The positive expression rates of PKM2 and HIF-1α in lymph node metastasis laryngeal carcinoma tissues were 90.00% and 100.00%, respectively. The positive rates of PKM2 and HIF-1α in laryngeal carcinoma without lymph node metastasis were 51.85% (P < 0.05). The positive rates of HIF-1α in stage Ⅰ-Ⅱ and stage Ⅲ-Ⅳ laryngeal carcinoma were 53.85% and 90.91%, respectively. There was significant difference between the two groups (P <0.05). The expressions of PKM2 and HIF-1α were not related to the age of patients with laryngeal cancer and the amount of smoking (P> 0.05). The expression of PKM2 and HIF-1α in laryngeal carcinoma cells was correlated (r = 0.476, P <0.01). Conclusion: The expressions of PKM2 and HIF-1α are closely related to the proliferation, invasion and metastasis of laryngeal carcinoma cells. The detection of PKM2 and HIF-1α may be used as biological indicators to provide a theoretical basis for the diagnosis and screening of laryngeal carcinoma.