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以乙肝表面抗原基因疫苗为模型， 采用肌肉注射、腹腔注射、表皮划痕三种免疫途径和不同剂量的裸露质粒ＤＮＡ免疫小鼠， 以ＥＬＩＳＡ方法检测小鼠血清中抗ＨＢｓＡｇ 抗体变化。实验结果表明表皮划痕方式免疫小鼠免疫反应阳性率高，且在相同剂量下其抗体滴度水平最高； 肌肉注射方式免疫小鼠， 抗体反应可维持较长时间； 腹腔注射方式免疫小鼠， 抗体反应产生快， 但维持时间较短。
The hepatitis B surface antigen gene vaccine was used as a model, and the mice were immunized with naked plasmid DNA by intramuscular injection, intraperitoneal injection and epidermal scratching, and the anti-HBsAg antibody levels in serum were detected by ELISA. The experimental results showed that the epidermal scratch immunization mice immunized with high positive rate, and the antibody titer at the same dose highest level; intramuscular immunization of mice, the antibody response can be maintained for a long time; intraperitoneal injection of immunized mice, Antibody response produces faster but shorter maintenance time.