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用10只存活5d的单侧后肢备用根猫(切除L1~L5、L7~S2背根节,保留L6背根),取手术侧(实验组)和非手术侧(对照组)脊髓Ⅱ板层组织块及提取液分别与Hamburger35期鸡胚背根节进行悬滴培养,并以不加植块的背根节培养作参照。比较各组在同一观测时间的差异.结果:(1)各组背根节从培养24h到48h,其神经突起均明显增长;(2)同一观测时间内,对照组与参照组的神经突起少而短,从背根节迁出的细胞较多,而实验组神经突起多且长,迁出的细胞较少;(3)在两个观测时间,植块与提取液培养的实验组背根节神经突起平均长度均显著长于对照组者,而对照组与参照组背根节神经突起长度的差异无显著性.表明部分腰骶背根切除猫,其脊髓Ⅱ板层及其提取液的促神经突起生长活性增强.
Ten surviving single-hind limb radish (5 days old) were randomly divided into three groups: one (1) L5-L5, one L7-S2 dorsal root and L6 dorsal root. Tissue blocks and extracts were suspended suspension culture with Hamburger35 chick embryo dorsal root section, and with no planting of dorsal root ganglia for reference. Compare the differences between groups in the same observation time. Results: (1) The dorsal root ganglia in each group grew significantly from 24h to 48h after culture. (2) Within the same observation time, the neurites in the control group and the reference group were less and shorter and migrated from dorsal root ganglion There were more cells in the experimental group, more neurites in the experimental group and less cells in the experimental group. (3) The mean length of dorsal root ganglion neurons in the experimental group cultured with explant and extract were significantly longer than those in the control group However, there was no significant difference in the length of dorsal root ganglion between the control group and the reference group. Show that some lumbosacral dorsal rhizotomy cats, the spinal cord layer Ⅱ and its extracts of the neurite growth enhancing activity.