论文部分内容阅读
在一些系统中,癌基因的激活导致肿瘤的形成,而癌基因的激活是通过病毒转导,病毒促进子的插入和染色体易位三者之一来完成的。激活的癌基因可用DNA转染NIH3T3成纤维细胞后是否有集落形成来检测。在这种类型的实验中,最早是用鸟类白血病病毒(ALV)来诱导鸡B细胞淋巴瘤,其中的C-myc由逆转录病毒促进子的插入而激活。使人感到惊奇的是,具转化功能的DNA序列不是C-myc,而是一未知的癌基因。这种癌基因被称为ChBlym-1或简称Blym。用六种Burkitt淋巴瘤的DNA来转染NIH3T3细胞时发现有相同的癌基因,而另一人类
In some systems, the activation of oncogenes leads to the formation of tumors, and the activation of oncogenes is accomplished through one of three mechanisms: viral transduction, viral facilitator insertion, and chromosomal translocation. Activated oncogenes can be detected using DNA-transfected NIH3T3 fibroblasts after colony formation. In this type of experiment, avian leukosis virus (ALV) was first used to induce chicken B-cell lymphoma, in which C-myc is activated by retroviral promoter insertion. Surprisingly, the DNA sequence with transformation is not C-myc but an unknown oncogene. This oncogene is called ChBlym-1 or Blym for short. NIH3T3 cells were transfected with DNA from six Burkitt’s lymphomas and found the same oncogene, while another human