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目的:建立以切换波长的方法同时测定升麻中咖啡酸、升麻素苷、阿魏酸、异阿魏酸、升麻素5种成分含量的方法。方法:采用反相高效液相色谱法,Agilent Eclipse XDB-C18(4.6 mm×150 mm,5μm)色谱柱;流动相:乙腈-0.1%磷酸水溶液(13:87);检测波长:0～7 min,323 nm;7～12 min,254 nm;12～17 min,316 nm;17～20 min,254 nm;柱温:40℃;流速:1.0 mL.min-1;进样量:10μL。结果:不同商品地的升麻中5种成分的含量差异显著,升麻素苷和升麻素在部分样品中未检出。结论:切换波长法可用于升麻中5种成分含量的同时测定,为升麻质量评价提供了科学依据。
OBJECTIVE: To establish a method for the simultaneous determination of five components of caffeic acid, ciminolate, ferulic acid, isoferulic acid and cimicifuguin by using wavelength switching method. Methods: The mobile phase was acetonitrile-0.1% phosphoric acid solution (13:87) using reversed-phase high performance liquid chromatography with Agilent Eclipse XDB-C18 (4.6 mm × 150 mm, , 323 nm, 7-12 min, 254 nm, 12-17 min, 316 nm, 17-20 min, 254 nm, column temperature: 40 ° C, flow rate: 1.0 mL.min-1, and injection volume: 10 μL. Results: The contents of five components in different commercial areas were significantly different. Cimicifuga and cola were not detected in some samples. Conclusion: The wavelength switching method can be used for the simultaneous determination of five components in cohosh, providing a scientific basis for the quality evaluation of cohosh.