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目的 :探讨去铁处理对实验性大鼠免疫性肝损伤的影响。 方法 :将 4 8只Wistar大鼠随机分成单纯对照组、单纯肝损伤组、放血对照组、放血肝损伤组、去铁胺 (DFO)对照组、DFO肝损伤组 6组 ,通过放血或DFO疗法制做低铁模型 ,采用卡介苗加脂多糖诱导法复制免疫性肝损伤模型。检测血清铁 (SI)、转铁蛋白 (TRF)、总蛋白 (TP)含量及天冬氨酸转氨酶 (AST)活性 ,肝组织中丙二醛 (MDA)含量、超氧化物歧化酶 (SOD)活性和肝铁含量 (HIC)的变化。 结果 :与对照组比较 ,DFO对照组及放血对照组SI有降低趋势 ,但差异不显著 ;DFO对照组TRF增高 ,放血对照组TRF降低 ;肝损伤后SI水平和TRF均显著下降 ,AST均增高 ,TP均降低 ;DFO损伤组和放血损伤组AST活性增高幅度和TP含量的降低幅度均小于单纯肝损伤组。各组动物肝组织铁含量无显著差异。与对照组比较 ,DFO对照组及放血对照组MDA均显著降低 ,SOD活性仅有下降趋势。各损伤组与其相应对照组比较 ,MDA含量均增高 ,SOD活性均降低。 结论 :去铁处理降低铁负荷 ,可以改善肝损伤程度
Objective: To investigate the effects of deferox treatment on immunological liver injury in experimental rats. Methods: Forty eight Wistar rats were randomly divided into control group, liver injury group, blood loss control group, bleeding liver injury group, DFO control group and DFO liver injury group. Making low-iron model, the use of BCG plus lipopolysaccharide induced immune liver injury model. Serum iron (SI), transferrin (TRF), total protein (TP) and aspartate aminotransferase (AST) activity, liver tissue malondialdehyde (MDA) content, superoxide dismutase (SOD) Activity and liver iron content (HIC) changes. Results: Compared with the control group, the SI in the DFO control group and the bleeding control group showed a trend of decrease, but the difference was not significant. The TRF of the DFO control group was increased and the TRF of the bleeding control group was decreased. The levels of SI and TRF were significantly decreased and AST were increased , TP decreased. The increase of AST activity and the decrease of TP content in DFO injury group and bleeding injury group were less than that in liver injury group. There was no significant difference in the content of iron in the liver of each group of animals. Compared with the control group, the MDA in the DFO control group and the bleeding control group decreased significantly, while the SOD activity decreased only. Compared with the corresponding control group, the content of MDA and the activity of SOD decreased in each injury group. Conclusion: Deferrization can reduce the iron load and improve the degree of liver injury