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为了解假两性畸型（Ｔｆｍ）小鼠睾丸胆固醇侧链裂解酶（Ｐ４５０ｓｃｃ）ｍＲＮＡ的水平及影响其转录的机理，用反转录－多聚酶链反应比较了５ｄ、２０ｄ、２５ｄ和３０ｄ正常小鼠、Ｔｆｍ小鼠及３０ｄ手术隐睾小鼠睾丸Ｐ４５０ｓｃｃ的ｍＲＮＡ水平，正常小鼠在５～２０ｄ时，Ｐ４５０ｓｃｃｍＲＮＡ水平较低，从２５ｄ起明显增高，至３０ｄ时ｍＲＮＡ水平是５ｄ时的７５倍；Ｔｆｍ小鼠在５ｄ时，Ｐ４５０ｓｃｃｍＲＮＡ水平稍高于正常小鼠，但随后直至３０ｄ其水平未见明显增高，在３０ｄ时，Ｐ４５０ｓｃｃｍＲＮＡ总量低于正常小鼠１／１０；３０ｄ手术隐睾小鼠的Ｐ４５０ｓｃｃｍＲＮＡ水平低于正常３０ｄ小鼠１／２，高于Ｔｆｍ小鼠５倍。结果提示，２５ｄ的Ｌｅｙｄｉｇ细胞功能的形成需要受体介导的雄激素作用，而Ｔｆｍ小鼠由于编码雄激素受体基因突变，间接导致青春期Ｐ４５０ｓｃｃｍＲＮＡ的低水平。
To investigate the level of P450scc mRNA in testes and the mechanism of its transcription, we compared the normal mice at 5d, 20d, 25d and 30d with reverse transcription polymerase chain reaction (RT-PCR) Tfm mice and 30d cryptorchid testis testes P450scc mRNA levels in normal mice at 5 ~ 20d, P450sccmRNA levels were lower, from 25d significantly increased, by the 30d mRNA level is 75d after 5d; Tfm small At 5 days, the level of P450sccmRNA was slightly higher than that of normal mice, but the level of P450sccmRNA was not significantly increased up to 30th day. The total amount of P450sccmRNA was less than 1/10 of that of normal mice at 30 days. P450sccmRNA level Lower than normal 30d mice 1/2, 5 times higher than Tfm mice. The results suggest that receptor-mediated androgens are required for the formation of Leydig cell function on day 25d, whereas Tfm mice indirectly lead to low P450sccmRNA levels due to mutations in androgen receptor genes.