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目的 了解在广东省受血及献血者中发现的HIV 1亚型的流行规律及其与国际参考株的同源性。方法 应用套式聚合酶链反应 (PCR)对 1 4例采自于广东省HIV 1抗体阳性的受血者或献血者淋巴细胞富集液的核酸样品进行扩增 ,并使用ABI377型测序仪对扩增产物测序后 ,对其ENV基因C2 V3段的核酸序列进行比较分析。结果 1 4份血样中 ,7份为泰国B’亚型 ,与国际参考株RL4 2的距离最近 ,基因离散率为 (6 0 82± 2 6 0 7) % ,组内离散率为 (5 96 3± 2 383) % ;4份为AE重组亚型 ,与国际参考株TH 90 CM2 4 0最近 ,基因离散率为 (8 90 0± 1 830 ) % ,组内离散率为 (1 3 81 0± 1 31 7) % ;2份为 0 7 BC重组亚型 ,与国际参考株CN 97 C5 4A最近 ,基因离散率为 (4 1 5 5± 1 2 2 3) % ,组内基因距离为 6 36 % ;1份为 0 8 BC重组亚型 ,与国际参考株 97CNGX 9F最近 ,基因距离为 0 97%。结论 本次检测的广东省受血及献血者HIV 1以泰国B’亚型为主 ,也存在主要在性途径感染人群中流行的AE重组亚型和吸毒者中流行的 0 7 BC、0 8 BC重组亚型。
Objective To understand the prevalence of HIV subtype 1 found in blood donors and blood donors in Guangdong Province and its homology with the international reference strains. Methods Nucleic acid samples from 14 blood donors or donors’ lymphocyte-enriched blood collected from Guangdong HIV-1 antibody were amplified by nested polymerase chain reaction (PCR) and sequenced by ABI377 After sequencing of the amplified product, the nucleotide sequence of the C2 V3 of ENV gene was compared. Results Among the 14 blood samples, 7 were B ’subtype of Thailand. The distance from the reference strain RL4 2 was (6 0 82 ± 2 6 0 7)%, and the in-group scatter rate was (5 96 3 ± 2 383)%, 4 were AE subtypes, and the most recent genetic comparison with the international reference strain TH 90 CM2 4 0 was 8 90 0 ± 1 830% ± 1 31 7)%; 2 were 0 7 BC recombinant subtypes. The closest genetic distance to the international reference strain CN 97 C5 4A was (4 1 5 5 ± 1 2 2 3)%, and the intragenomic distance was 6 36%; 1 copy was 0 8 BC recombinant subtype, with the international reference strain 97CNGX 9F recently, the genetic distance was 97%. CONCLUSION: The majority of HIV-1 donors and blood donors in Guangdong province are Thai B ’subtypes. There are also prevalences of 0 7 BC, 0 8 prevailing among AE subtypes and drug users, mainly among the sexually transmitted infections BC recombinant subtype.