论文部分内容阅读
目的：探讨女性生殖道人乳头瘤病毒感染与宫颈癌前病变的关系。方法：对２５３例妇科感染患者用ＰＣＲ方法检测ＨＰＶ分型，同时对７８例不同宫颈病变组织行ＨＰＶ１６、１８型原位杂交，并在邻近切片用免疫组化方法检测增殖细胞核抗原。结果：１）女性生殖道ＨＰＶ感染率，由慢性宫颈炎→假性湿疣→疣样病变→尖锐湿疣→ＣＩＮ→宫颈癌，随宫颈病变程度的加重而逐渐升高，ＩＳＨ阳性杂交信号的分布与ＨＥ染色中挖空细胞的分布相一致。２）ＨＰＶ１６、１８型感染率及ＰＣＮＡ表达率均随宫颈病变程度的加重呈升高趋势。挖空细胞核呈ＰＣＮＡ阳性反应，与ＩＳＨ阳性杂交信号出现的部位相一致。结论：ＨＰＶ感染可使宫颈上皮细胞获得较高的增殖活性，也可能通过促进细胞的过度增殖活性而致癌变。
Objective: To investigate the relationship between human reproductive tract human papillomavirus infection and cervical precancerous lesions. Methods: HPV genotypes were detected by PCR in 253 patients with gynecological infection. HPV16,18 type in situ hybridization was performed on 78 cases of different cervical lesions. The proliferating cell nuclear antigen was detected by immunohistochemistry in adjacent sections. Results: 1) The prevalence of HPV infection in female genital tract was gradually increased from chronic cervicitis → pseudocondaciarum → wart-like lesions → genital warts → CIN → cervical cancer with the severity of cervical lesions. The distribution of ISH positive hybridization signal and The distribution of hollowed cells in HE staining was consistent. 2) HPV16,18 infection rate and PCNA expression rate were increased with the severity of cervical lesions increased. The knockout nucleus was positive for PCNA, consistent with the appearance of the ISH-positive hybridization signal. Conclusion: HPV infection can cause cervical epithelial cells to obtain higher proliferative activity, and may also cause carcinogenesis by promoting cell hyperproliferative activity.