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目的对比研究链霉素纳米制剂与普通制剂——硫酸链霉素注射液在小鼠体内经肠胃外给药时的抗结核活性。方法雌性BALB/c小鼠尾静脉感染结核分枝杆菌H37Rv 1×107CFU。感染后第2 d开始按照空白对照组、阳性对照组、比较对照组和纳米链霉素SM-NP BSA组、纳米链霉素SM-NP CRM组的剂量和频率分别腹腔注射给药,给药至感染后28 d。在感染的14、28、56 d分别处死每组4只小鼠进行脾、肺活菌计数。结果SM-NP CRM组在整个实验期间的存活率是100%,SM-NP BSA组第28 d和第56 d的存活率分别为83%和75%。治疗14 d时,比较对照组小鼠脾、肺的菌量较阳性对照组大,而SM-BSA与SM-CRM组小鼠脾、肺的活菌数较阳性对照组降低;治疗4周时,各组的活菌数与2周时相比没有明显变化,但SM-NP BSA与SM-NP CRM组的脾、肺活菌数均比比较对照组低,差异有统计学意义。感染8周时,SM-NP BSA、SM-NP CRM组肺的活菌数量仍比阳性对照组小,差异有统计学意义。结论纳米链霉素制剂SM-NP BSA、SM-NP CRM与普通链霉素注射液相比,在给药剂量与给药频率降低时,在小鼠急性结核病感染模型中表现的抗菌活性仍与阳性对照相当,值得进一步研究。
Objective To compare the antituberculous activity of streptomycin nano-preparation and common preparation-streptomycin sulfate injection when administered parenterally in mice. Methods Female BALB / c mice were infected with Mycobacterium tuberculosis H37Rv 1 × 107CFU through the tail vein. The dose and the frequency of the SM-NP BSA group and the Nano-streptomycin SM-NP BSA group and the control group, the SM-NP BSA group and the Nano-streptomycin SM-NP CRM group were respectively administered by intraperitoneal injection and administration 28 d after infection. Infected 14,28,56 d were sacrificed in each group of 4 mice spleen, lung viable count. Results The survival rate of the SM-NP CRM group was 100% throughout the experiment, and the survival rates of the SM-NP BSA group at the 28th day and the 56th day were 83% and 75%, respectively. On the 14th day after treatment, the spleens and lungs in the control group were larger than those in the positive control group, while the viable cells in the spleen and lung of the SM-BSA and SM-CRM groups were lower than those in the positive control group. After 4 weeks of treatment , The number of viable cells in each group did not change significantly compared with that of 2 weeks. However, the viable count of spleen and lung of SM-NP BSA and SM-NP CRM groups were lower than that of the control group, the difference was statistically significant. At 8 weeks of infection, the number of viable cells in the lung of the SM-NP BSA and SM-NP CRM groups was still smaller than that of the positive control group, and the difference was statistically significant. Conclusion Compared with streptomycin injection, the antibacterial activity of SM-NP BSA and SM-NP CRM in mouse model of acute tuberculosis infection was still lower than that of the control group Positive control quite worthy of further study.