锌缺乏对小鼠周围血淋巴细胞增殖反应的抑制效应

• 作 者: 李刚
• 机 构: 海南医学院蛋白质研究室
• 出 处: 海南大学学报(自然科学版)
• 发表日期: 1994年01期
• 关键词: 锌缺乏 抑制效应 有丝分裂原 小鼠体重 食物摄入 缺锌 刺激反应 增殖反应 细胞分化 血淋巴

本实验测定锌缺乏时小鼠周围血淋巴细胞（ＰＢＬ）对有丝分裂ＣｏｎＡ刺激反应的改变。当用不同锌含量的合成饲料饲养Ａ／Ｊ小鼠２８天后，缺锌组小鼠体重和总食物消耗明显减少，体重仅为对照组的６８％。当用５μｇＣｏｎＡ／ｍｌ刺激ＰＢＬ时，中度和重度缺锌组细胞３￣Ｈ－ＴｄＲ掺入显著减少，分别为对照组的５２％和３１％，而限制组与对照组相比无明显改变。结果说明：缺锌时周围血淋巴细胞免疫功能受到严重损害，而不是由于食物摄入不足所致。

This experiment measured zinc deficiency in mice peripheral blood lymphocytes (PBL) changes in response to mitosis ConA stimulation. After 28 days of feeding A / J mice with synthetic diets containing different amounts of zinc, the body weight and total food consumption of the zinc-deficient mice were significantly reduced, weighing only 68% of the control. When PBLs were stimulated with 5 μg CONA / ml, 3H-TdR incorporation was significantly reduced in moderate and severe zinc-deficient groups, 52% and 31%, respectively, of the control group, with no significant change in the restricted group compared to the control group. The results show that: zinc deficiency peripheral blood lymphocytes immune function was severely damaged, not due to inadequate food intake due.