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将精子洗净后用尼龙网过滤,除去凝集的精子,连续注入无载体电泳装置,用光学检查系统记录精子的分离状态。精子向阳极泳动约30分钟可见到两个高峰(泳动度为1.07和0.41)。在两峰之间可见一小峰,显微镜观察结果为精液中所含的上皮碎片、白细胞和脂肪滴等。用阿地平芥子荧光染色法计算Y精子比率时见,泳动度快的近阳极侧的峰中无F小体,只含X精子,相反泳动度慢的近阴极侧的峰中Y精子的比率为83～89%,可见能较好地分离X、Y精子。细胞表面的负电与细胞膜磷脂及表面糖蛋白中的唾液酸和硫酸化糖的负电密切相关。X、Y精子
The sperm were washed with nylon mesh filtration, removal of agglutinated sperm, continuous injection of carrierless electrophoresis device, the optical inspection system to record the separation of sperm. Sperm swimming to the anode about 30 minutes to see two peaks (mobility of 1.07 and 0.41). Visible a small peak between the two peaks, the microscope results of the epithelial debris contained in semen, leukocytes and fat droplets and so on. When the Y-sperm ratio was calculated by the staphyloferrin staining, it was found that there was no F-body on the near-anodic peak with high mobility, only X-containing sperm, and on the other hand, Y sperm The ratio of 83% to 89% shows that X and Y sperm can be separated well. The negative charge on the cell surface is closely related to the negative charges of sialic acid and sulfated saccharide in cell membrane phospholipids and surface glycoproteins. X, Y sperm