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目的探讨阿魏酸钠对5/6肾切除大鼠肾脏病变的保护作用。方法大鼠随机分为4组,假手术组(Sham组),肾切除组(N X组),N X+阿魏酸钠组(SF组),N X+依那普利(E组)。8周后检测大鼠血压、24h尿蛋白定量、血肌酐、BU N,肾皮质内皮素-1(ET-1)和一氧化氮(N O)等,观察肾组织病理改变,并应用R T-PC R的方法检测肾皮质内T G F-β1m R N A的表达;W estern蛋白印迹方法检测TG F-β1蛋白质的表达。结果术后大鼠出现明显的尿蛋白[(201.2±4.1)m g/24h],肾皮质ET-1含量显著升高,且有明显的肾小球硬化,而阿魏酸钠和依那普利均能减少尿蛋白[阿魏酸钠(78.1±3.1)m g/24h,依那普利(65.9±6.3)m g/24h,与假手术组比,P<0.01],减少肾皮质ET-1含量和肾小球硬化指数。肾大部切除后肾皮质T G F-β1m R N A及其蛋白的表达明显升高,用药组上述指标明显降低(SF组和E组与N X组相比,P<0.01)。结论在5/6肾大部切除模型中,阿魏酸钠对5/6肾大部切除大鼠肾脏有明显的保护作用,其机制可能其减少肾脏ET-1的生成,抑制TG F-β1的表达有关。
Objective To investigate the protective effect of sodium ferulate on renal lesions in 5/6 nephrectomized rats. Methods The rats were randomly divided into 4 groups: sham operation group (Sham group), nephrectomy group (N X group), N x + sodium ferulate group (SF group) and Nx + enalapril group (E group). After 8 weeks, the blood pressure, 24h urinary protein, serum creatinine, BU N, ET-1 and NO were measured to observe the pathological changes of renal tissue. PC R method was used to detect the expression of TG F-β1m RNA in renal cortex. Western blotting was used to detect the expression of TG F-β1 protein. Results Significant urine protein ([(201.2 ± 4.1) mg / 24h] was observed in the postoperative rats. The content of ET-1 in the renal cortex was significantly increased and there was significant glomerulosclerosis. However, sodium ferulate and enalapril (78.1 ± 3.1) mg / 24h and enalapril (65.9 ± 6.3) mg / 24h respectively, P <0.01 compared with the sham-operation group) and reduce the content of ET-1 in renal cortex And glomerulosclerosis index. The expression of T G F -β1 RAN and its protein in renal cortex was significantly increased after subtotal nephrectomy, and the above parameters were significantly decreased in the treatment group (P <0.01 compared with the N X group in SF group and E group). Conclusion In 5/6 subtotal nephrectomy model, sodium ferulate has a protective effect on the kidneys of 5/6 subtotal nephrectomized rats. The mechanism may be that it reduces the production of ET-1 and inhibits the expression of TG F-β1 Related to the expression.