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综合shRNA介导的基因沉默和全基因组表达芯片杂交技术,筛查了肺腺癌肿瘤干细胞(cancer stem cells,CSC)中靶向灭活Nanog多潜能基因后的差异表达基因。发现Nanog沉默引起了1 605个基因差异表达,其中95个基因属于受Nanog转录调控的靶基因。对差异表达基因进行KEGG分析后发现,Nanog的一个重要功能是转录抑制蛋白酶体编码基因表达。此外,研究还发现在此类细胞的特征性基因表达谱中,25个基因的表达受Nanog调控,包括15个基因在Nanog沉默后表达上调和10个基因表达下调。上述研究结果揭示,Nanog多潜能转录因子在肺腺癌的CSC中具有转录调控功能。
The shRNA-mediated gene silencing and genome-wide expression microarray hybridization were used to screen differentially expressed genes that targeted and inactivated Nanog pluripotency gene in lung adenocarcinoma cancer stem cells (CSC). Nanog silencing was found to cause a differential expression of 1 605 genes, of which 95 genes belonged to the target gene under the transcriptional regulation of Nanog. KEGG analysis of differentially expressed genes revealed that an important function of Nanog was transcriptional repression of proteasome-encoding gene expression. In addition, the study also found that in the characteristic gene expression profiles of such cells, the expression of 25 genes is regulated by Nanog, including 15 genes upregulated after Nanog silencing and 10 genes downregulated. The above results revealed that Nanog pluripotency transcription factor has a transcriptional regulatory function in CSC of lung adenocarcinoma.