【摘 要】
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AIM: To investigate the effect of lithium on proliferation of esophageal cancer (EC) cells and its preliminary mechanisms.METHODS: Eca-109 cells were treated wi
【机 构】
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Department of Pathology
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AIM: To investigate the effect of lithium on proliferation of esophageal cancer (EC) cells and its preliminary mechanisms.METHODS: Eca-109 cells were treated with lithium chloride, a highly selective inhibitor of glycogen synthase kinase 3β (GSK-3β), at different concentrations (2-30mmol/L) and time points (0,2,4,6 and 24h). Cell proliferative ability was evaluated by 3-(4,5-Dlmethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell cycle distribution was examined by flow cytometry. Expressions of p-GSK-3β,15-catenin, cyclin B1, cdc2 and cyclin D1 protein were detected by Western blotting, and the subcellular localization of β-catenin was determined by immunofluorescence. The Mrna level of cylin B1 was detected by reverse transcription polymerase chain reaction (RT-PCR).RESULTS: Lithium could inhibit the proliferation of Eca-109 cells. Lithium at a concentration of 20mmol/L lithium for 24h produced obvious changes in the distribution of cell cycle, and increased the number of cells in G2/M phase (P
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