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目的 建立化学发光法检测药物光毒性的模型。方法 实验中将还原型辅酶I(NADH)与待测药物混合后置于UVA光源 (发射光谱 31 0nm～ 4 0 0nm ,峰值位于 35 0nm处 )下照射 ,一定剂量后加入化学发光剂鲁米诺 ,计数化学发光值 ,以发光值反映光毒反应强度。结果 经不同剂量UVA照射后 ,各种药物和蒸馏水样品发光值均有不同程度的升高 ,发光值与UVA剂量呈直线正相关关系。统计学检验结果显示 :与蒸馏水样品相比 ,吡哌酸、强力霉素和灰黄霉素样品发光值差别有显著性。结论 化学发光法检测到吡哌酸、强力霉素和灰黄霉素的光毒性 ,光毒反应强度在一定范围内随UVA剂量增加而增强。
Objective To establish a model of chemiluminescence detection of phototoxicity of drugs. Methods In the experiment, the reduced coenzyme I (NADH) was mixed with the drug under test and then placed in the UVA light source (emission spectrum 31 0-400 nm, the peak at 35 0 nm) under irradiation, after a certain dose of luminol chemiluminescence , Count the value of chemiluminescence to reflect the light intensity of phototoxic reaction intensity. Results After different doses of UVA irradiation, the luminescence values of various drugs and distilled water samples all increased to some extent. There was a linear positive correlation between luminescence and UVA dose. Statistical test results show that, compared with distilled water samples, the differences in luminescence values of pipemidic acid, doxycycline and griseofulvin samples were significant. Conclusion The phototoxicity of pipemidic acid, doxycycline and griseofulvin was detected by chemiluminescence method. The phototoxic reaction intensity increased with the increase of UVA dose within a certain range.