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Objective To describe the successful use of preimplantation genetic d iagnosis to assess the prevalence of meiotic errors after oocyte cryopreservation in an o ocyte donation cycle. Design Case report. Setting Private IVF center. Patient(s) A 42.6-year old patient with ovarian failure. Intervention(s)A donor oocyte IV F cycle with cryopreservation of oocytes followed by thaw, fertilization of oocy tes, preimplantation genetic diagnosis for selective aneuploidy, and ET. Main ou tcome measure(s) Preimplantation genetic analysis of chromosomes 13,16,18, 21,22 , X, and Y with fluorescence in-situ hybridization. Result(s)-The recipients initial serum β-hCG level was 196 mIU/mL 15 days after oocyte retrieval. An i nitial ultrasound at the sixth week of gestation revealed two gestational sacs. A second ultrasound 1 week later showed a monochorionic twin in sac A and a sing leton pregnancy in sac B. Fetal cardiac activity was visualized for all gestatio ns. Conclusion(s) This case illustrates the feasibility of cryopreservation of d onor oocytes combined with preimplantation genetic diagnosis for clinical use in those settings where there may be an increased risk of spindle-related abnorma lities.
Objective To describe the successful use of preimplantation genetic d iagnosis to assess the prevalence of meiotic errors after oocyte cryopreservation in an o ocyte donation cycle. Design Case report. Setting Private IVF center. Patient (s) A 42.6-year old patient with ovarian failure . Intervention (s) A donor oocyte IV F cycle with cryopreservation of oocytes followed by thaw, fertilization of oocy tes, preimplantation genetic diagnosis for selective aneuploidy, and ET. Main ou tcome measure (s) Preimplantation genetic analysis of chromosomes 13, 16, 18, 21, 22, X, and Y with fluorescence in-situ hybridization. Result (s) -The recipient’s initial serum β-hCG level was 196 mIU / mL for 15 days after oocyte retrieval. Ani nitial ultrasound at the sixth week of gestation revealed two gestational sacs. A second ultrasound 1 week later showed a monochorionic twin in sac A and sing leton pregnancy in sac B. Fetal cardiac activity was visualized for all gestatio ns. Conclusion (s) This case illustra tes the feasibility of cryopreservation of d onor oocytes combined with preimplantation genetic diagnosis for clinical use in those settings where there may be an increased risk of spindle-related abnorma lities.