论文部分内容阅读
为了探讨力竭运动对大鼠心脏心钠素表达的影响 ,采用 4月龄雄性 SD大鼠 42只 ,体重 2 2 0 g～ 36 0 g,随机分为 1正常对照组 (A组 )、2有训练游泳力竭组 (B组 )、3无训练游泳力竭组 (C组 )。每组各 14只。灌注后取出整心 ,用免疫组化 ABC法和图像分析技术以及透射电镜实验 ,显示和观察大鼠心肌心钠素的表达和心钠素电子致密颗粒的变化。结果是 :心钠素免疫阳性反应为 :右心耳 >右心房 >左心房 >右心室近心房处。与 A组相比 ,B组大鼠心房肌心钠素阳性反应明显增强 ,心钠素电子致密颗粒大量增加 ;C组大鼠心房肌心钠素表达明显减少 ,仅右心房肌细胞有少量心钠素颗粒。两组大鼠心肌组织和细胞超微结构出现程度不同的损伤性变化 ,以 C组大鼠心肌细胞结构的损伤性改变更明显。结果表明 :力竭运动可对大鼠心肌细胞的结构和心钠素表达产生损伤性影响。
In order to investigate the effect of exhaustive exercise on the expression of atrial natriuretic peptide (APA) in rat hearts, 42 male 4-month-old male Sprague-Dawley rats weighing 220-260 g were randomly divided into 1 group (A group), 2 Training swimming exhaustive group (B group), 3 untrained swimming exhaust group (C group). Each group of 14. The hearts were removed after perfusion, and the expression of atrial natriuretic peptide and the changes of atrial natriuretic peptide were observed and analyzed by immunohistochemical ABC, image analysis and transmission electron microscopy. The result is: atrial natriuretic peptide positive reaction: right atrial appendage> right atrium> left atrium> right ventricle near the atrium. Compared with group A, the atrial natriuretic peptide positive reaction in group B was significantly increased, and the number of atrial natriuretic peptide increased significantly. The atrial natriuretic peptide expression was significantly decreased in group C, and only a small amount of heart was found in the right atrial myocytes Sodium particles. The ultrastructural changes of myocardium and cells in the two groups showed different degrees of damage, and the changes of myocardial cell structure in C group were more obvious. The results showed that exhaustive exercise exerted a damaging effect on the structure of cardiac myocytes and the expression of atrial natriuretic peptide.