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目的研究维生素D受体(vitamin D receptor,VDR)基因多态性在老年男性中的分布, 并进一步研究其与骨密度的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR- RFLP)方法,分析145例老年男性的VDR基因型,同时用双能X线吸收法测定腰椎及髋部骨密度。结果 VDR基因型分别为BB,0．014;Bb,0．117;bb,0．869。骨质疏松组与非骨质疏松组之间VDR基因型分布频率的差异无显著性(P>0．05)。比较各基因型组的骨密度,bb组及 Bb组只有在股骨颈处显示出BMD均低于BB组,差异有显著性(P<0．05),其它部位,三个基因型组的BMD均差异无显著性(P>0．05)。结论老年男性VDR基因型分布频率与某些西方国家人群分布不同,其VDR基因型与骨密度无明显相关性。VDR基因可能不是我们所研究群体 BMD的主要遗传基因。
Objective To investigate the distribution of vitamin D receptor (VDR) gene polymorphism in elderly males and further study its relationship with bone mineral density. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the genotypes of VDR in 145 elderly men. BMD of lumbar spine and hip were measured by dual-energy X-ray absorptiometry. Results The genotypes of VDR were BB, 0.014; Bb, 0.117; bb, 0.869 respectively. There was no significant difference in the distribution frequency of VDR between osteoporosis group and non-osteoporosis group (P> 0.05). BMD of each genotype group was significantly lower than that of BB group only in the femoral neck in group bb and group Bb (P <0.05). BMD in other sites and three genotypes There was no significant difference (P> 0.05). Conclusion The frequency distribution of VDR genotypes in elderly men is different from that in some Western countries. There is no significant correlation between VDR genotypes and bone mineral density. The VDR gene may not be the major gene for BMD in our study population.