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目的探讨葡萄糖调节蛋白78(GRP78)在肥胖及肥胖相关疾病中的作用。方法将30例40～60岁女性患者分为3组:正常对照组(NC组,BMI<23kg/m2),超重组(OW组,BMI<28kg/m2)和肥胖组(OB组,BMI>28kg/m2),每组10例。在其腹部择期手术时取其网膜脂肪组织,应用RT-PCR和Western-blot检测上述三组患者脂肪组织中GRP78的表达水平。结果 OB组GRP78mRNA与蛋白水平(GRP78/β-actin)显著高于对照组(P<0.05)。GRP78/β-actin与BMI呈正相关(r≥0.75,P<0.05)。结论 GRP78表达量与BMI呈正相关(GRP78蛋白及mRNA水平OB组>OW组>NC组)。GRP78可作为预测肥胖及T2DM和心血管疾病危险因素的指标之一。
Objective To investigate the role of glucose regulatory protein 78 (GRP78) in obesity and obesity-related diseases. Methods Thirty patients with 40-60 years old were divided into three groups: normal control group (NC group, BMI <23kg / m2), overweight group (OW group, BMI <28kg / m2) and obesity group (OB group, BMI> 28kg / m2), 10 cases in each group. At the time of elective surgery, the omentum was taken for adipose tissue. The expression of GRP78 in adipose tissue of the above three groups was detected by RT-PCR and Western-blot. Results The GRP78 mRNA and protein levels (GRP78 / β-actin) in OB group were significantly higher than those in control group (P <0.05). GRP78 / β-actin was positively correlated with BMI (r ≥ 0.75, P <0.05). Conclusion The expression of GRP78 is positively correlated with BMI (GRP78 protein and mRNA level OB group> OW group> NC group). GRP78 can be used as a predictor of obesity and T2DM and cardiovascular disease risk factors.