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传统的致畸实验方法操作复杂、费时费力,尤其在剥皮及摘取肝脏时极易将胎仔的肋骨、指、尾骨撕伤,如脱肋、断指、断尾,且标本很软,观察时不易拿取.直接影响实验观察的正确性,也不易作教学标本、长期保存。通过多年的实践,我们对原法作了几点改进,获得满意效果,提供同行参考: 一、孕鼠剖腹后,将取出的胎仔直接放入95％乙醇中浸泡3～5天后再摘取肝脏,此时的肝脏较硬,易与周围的组织脱离。 二、将取肝后的胎仔放入2％的KOH溶液中浸
The traditional method of teratogenic operation is complex, time-consuming and laborious, especially when peeling and removing the liver, it is very easy to tear the ribs, fingers and coccyx of the fetus, such as removing the ribs, Not easy to take.A direct impact on the correctness of experimental observations, it is not easy to make teaching specimens, long-term preservation. After years of practice, we make some improvements to the original method, to obtain satisfactory results, to provide peer reference: First, the pregnant rats Caesarean section, remove the fetus directly into 95% ethanol soak 3 to 5 days before the removal of the liver , At this time the liver is harder, and easily detached from the surrounding tissue. Second, the fetus after taking the liver into 2% KOH solution immersed