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目的:检测宫颈上皮内瘤样病变(Cervical intraepitheual neoplasia,CIN)及各期宫颈癌组织中端粒酶的含量,探讨端粒酶含量改变作为宫颈癌的早期诊断,治疗及术后复发依据的可能性。方法:采用异硫氰酸荧光素(Fluo- rescein isothiocyanate,FITC)间接荧光标记法对端粒酶进行标记后,流式细胞仪进行定量检测。以端粒酶表达率及端粒酶表达的荧光指数为指标进行统计学分析。以正常宫颈组织为对照。结果:端粒酶表达的百分率及荧光指教两指标在宫颈漫润癌,CIN及正常对照的组间差异显著,且有渐减规律;宫颈癌不同期别的组间差异显著。结论:端粒酶含量在癌前病变中已表现出显著升高,且其含量随肿瘤的发展而进行性升高,因此,端粒酶的定量检测有可能成为宫颈上皮内瘤样病变进展及宫颈癌预后判断的辅助指标。
Objective: To detect the content of telomerase in cervical intraepithelial neoplasia (Cervical intraepithelial neoplasia, CIN) and various stages of cervical cancer, and to explore the possible role of telomerase activity in the early diagnosis, treatment and recurrence of cervical cancer Sex. Methods: Telomerase was labeled by Fluorescein isothiocyanate (FITC) indirect fluorescent labeling method, and then detected by flow cytometry. The telomerase expression and telomerase expression of fluorescence index as a statistical analysis. Normal cervical tissue as a control. Results: There were significant differences between the two groups in the percentage of telomerase expression and the index of fluorescence in cervical diffuse carcinoma, CIN and normal control group, and there was a gradual decrease rule. There were significant differences in different stages of cervical cancer. CONCLUSION: Telomerase activity has been significantly increased in precancerous lesions, and its content increased progressively with the development of tumors. Therefore, the quantitative detection of telomerase may be the development of cervical intraepithelial neoplasia and Auxiliary indicators of cervical cancer prognosis.