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据《美国医学论坛报》1997年12月18日载Carroll报道,英国研究人员称,医师可通过对羊膜穿刺液及绒膜绒毛采样标本进行新方法检测,更快地诊断胎儿畸形。 英国伦敦大学学院研究员Sherlock博士采用定量荧光聚合酶链反应(QF-PCR)技术,在24小时内确定85份标本中82份有胎儿畸形。(Am J Obstet Gynecol 1997,117:899)该文另一作者、妇产科研究人员Pertl医师称,他们采用PCR技术是为节省时间(常规培养及染色方法需要2周)。Sherlock医师等人从羊水、胎儿组织及血液提取DNA标本,然后用多重PCR扩增染色体21、18、13及X特有的多态性小串重复标记物,并进行仔细检查。接受检查的胎儿中有4例是因超声检查时发现异常被转来接受羊膜穿刺检查的。新试验发现
According to a report by Carroll on December 18, 1997 in the Medical Tribune in the United States, British researchers said that doctors can diagnose fetal malformations more quickly by carrying out new tests on samples of amniocentesis and chorionic villus samples. Dr. Sherlock, a researcher at the University College London, UK, used quantitative fluorescent polymerase chain reaction (QF-PCR) to determine 82 fetal distortions in 85 of the specimens within 24 hours. (Am J Obstet Gynecol 1997, 117: 899) Another author, obstetrics and gynecology researcher Pertl MD, said they are using PCR to save time (two weeks for routine culturing and staining methods). DNA samples from amniotic fluid, fetal tissue and blood were extracted by Dr. Sherlock et al. And then multiplexed PCR was used to amplify chromosome 21, 18, 13 and X-specific small polymorphism repeat markers and to examine them carefully. Four of the fetuses under examination were referred for amniocentesis due to abnormal findings on sonography. New test found