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目的研究低氧诱导因子(HIF-1α)和核因子(NF-κB)在小鼠视网膜光损伤后的表达及低氧预适应对其表达的影响,探讨低氧预适应对视网膜光损伤的保护机制。方法将54只BALB/C小鼠随机分成单纯光照组、低氧预适应组和正常组,前2组又按光照后处死时间的不同分为6、12、36h和7d组。应用免疫组织化学技术检测小鼠视网膜组织HIF-1α和NF-κB蛋白的表达,并分析低氧预适应组与单纯光照组HIF-1α和NF-κB蛋白的表达差异。结果正常小鼠视网膜组织HIF-1α几乎不表达,NF-κB有微量表达。低氧预适应组与单纯光照组比较,HIF-1α的表达强度明显增强,而NF-κB的表达强度明显减弱,两组比较差异有统计学意义(P<0.01)。结论低氧预适应可能通过提高HIF-1α的表达有效抑制NF-κB信号通路,从而通过降低凋亡的发生而起到保护作用。
Objective To investigate the expression of hypoxia-inducible factor (HIF-1α) and nuclear factor (NF-κB) after retinal light injury in mice and the effect of hypoxic preconditioning on the expression of HIF-1α, and to explore the protective effect of hypoxic preconditioning on retinal photodamage mechanism. Methods Fifty-four BALB / C mice were randomly divided into light group, hypoxic preconditioning group and normal group. The first two groups were divided into 6, 12, 36h and 7d groups according to the different irradiation time. Immunohistochemistry was used to detect the expression of HIF-1αand NF-κB protein in the retina of mice, and the differences of HIF-1αand NF-κB protein expression between the hypoxic preconditioning group and the pure light group were analyzed. Results HIF-1α in retinal tissue of normal mice was almost not expressed, while NF-κB was slightly expressed. Hypoxia preconditioning group compared with the light group, HIF-1α expression intensity was significantly increased, while the expression of NF-κB decreased significantly, the difference between the two groups was statistically significant (P <0.01). Conclusion Hypoxic preconditioning may protect NF-κB signaling pathway by decreasing the expression of HIF-1α and thus decreasing apoptosis.