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共生菌Cardinium是烟粉虱Bemisia tabaci体内的一种内共生细菌,对烟粉虱的生物学特性有着重要的影响。本文利用PCR技术,首先对两种入侵的B、Q生物型以及一种本土的Cv生物型烟粉虱种群体内Cardinium的感染率进行了检测,研究了不同浓度的利福平抗生素与高温处理对Q型烟粉虱体内的Cardinium的灭活情况;进一步比较了Cardinium阳性与阴性Q型烟粉虱种群在发育、存活与繁殖等方面的差异。研究结果表明,生物型不同,其体内Cardinium的感染率也存在显著差异;B、Q和Cv型烟粉虱Cardinium的感染率分别为26.7%、91.6%与82.9%。三种浓度的利福平蔗糖溶液(0.5、1.0与2.0 mg/m L)对Q型烟粉虱体内Cardinium的灭活效果不同,考虑到灭活效果及对烟粉虱生命力的影响,以1.0 mg/m L的浓度连续饲喂7 d以上的灭活效果最佳;高温36-40℃连续处理3-9 d均不能去除Q型烟粉虱体内的Cardinium。与Cardinium阳性的烟粉虱种群相比,阴性的Q型烟粉虱种群发育延迟,世代存活率降低,后代雄性比例增加,单雌产卵量减少。研究结果对于全面了解Cardinium共生菌与烟粉虱之间的互作关系具有重要的价值。
Symbiotic Bacterium Cardinium is an endosymbiotic bacterium of Bemisia tabaci, which has an important effect on the biological characteristics of Bemisia tabaci. In this paper, the prevalence of Cardinium infection in two invasive B and Q biotypes and a native Cv biotype Bemisia tabaci population was detected by PCR. The effects of different concentrations of rifampicin and high temperature treatment Q-type B. tabaci Cardcard inactivation; further comparison of Cardinium positive and negative Q-biotype Whitefly populations in development, survival and reproduction differences. The results showed that the infection rates of Cardinium were also significantly different in different biotypes. The infection rates of Cardinium in B. tabaci Q and Cv were 26.7%, 91.6% and 82.9% respectively. Three concentrations of rifampicin sucrose solution (0.5, 1.0 and 2.0 mg / m L) of Cardiotoxicity in vivo Q-type B. tabaci inactivation effect is different, taking into account the inactivation effect and the impact on the viability of B. tabaci, to 1.0 The concentration of mg / m L was the best inactivation for more than 7 days, while the treatment of 36-45 ℃ for 3-9 d could not remove Cardinium in B. tabaci. Compared with the Cardinium-positive Bemisia tabaci population, the population of negative Q-biotype Bemisia tabaci was delayed, the generational survival rate decreased, the proportion of male offspring increased, and the number of ovipositional fecundity decreased. The research results have important value for understanding the interaction between Cardinium commensal bacteria and B. tabaci.