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目的筛选新的能够识别红内期约氏疟原虫17XL的TLRs。方法扩增并克隆Balb/c小鼠TLR1,TLR3,TLR5,TLR6,TLR7,TLR9,TLR11全长编码序列,并克隆至表达载体pcDNA3.1(+);搭建活化TLRs的NF-κB和IFN-β报告基因检测平台,并利用该平台系统筛选能够识别约氏疟原虫致死株17XL红内期超声刺激物的新TLRs。结果 NF-κB和IFN-β报告基因实验结果发现,红内期超声刺激物能够活化TLR2,TLR4,TLR9,但不能通过TLR3活化IFN-β,以及通过TLR5,TLR7,TLR9,TLR11活化NF-κB。结论除了能被TLR2(TLR2/TLR1,TLR2/TLR6),TLR4和TLR9识别外,17XL疟原虫红内期超声刺激物并不能被其他已知TLRs(TLR3,TLR5,TLR7,TLR11)所识别。
Objective To screen for new TLRs that recognize 17XL of Plasmodium yoelii in the red stage. Methods The full-length coding sequences of TLR1, TLR3, TLR5, TLR6, TLR7, TLR9 and TLR11 in Balb / c mice were amplified and cloned into the expression vector pcDNA3.1 (+ β reporter gene detection platform, and use the platform system to screen new TLRs that can identify the 17XL red-phase ultrasonic stimulus of the lethal strain of Plasmodium yoelii. Results The results of NF-κB and IFN-β reporter gene assays showed that red-phase ultrasound stimuli could activate TLR2, TLR4 and TLR9, but not IFN-β through TLR3 and NF-κB through TLR5, TLR7, TLR9 and TLR11 . Conclusion The 17XL malaria red-phase internal sonotrope can not be recognized by other known TLRs (TLR3, TLR5, TLR7, TLR11) except that it can be recognized by TLR2 (TLR2 / TLR1, TLR2 / TLR6), TLR4 and TLR9.