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:目的 建立固定中性粒细胞 ELISA ,分析血清ANCA对UC的诊断价值 .方法 采用固定中性粒细胞 ELISA法检测 2 5份溃疡性结肠炎血清、2 6份细菌性结肠炎血清、15份混合类型腹泻血清和 5 0份正常对照血清中的ANCA ,对各组血清的吸光度A值进行统计分析 .结果 UC组ANCA与其它对照组ANCA之间的差异非常显著 (P <0 .0 0 5 ) .UC组A值超过正常对照组A值 2SD以上病例达 80 % ,诊断特异性达 89% .结论 UC血清中存在有诊断价值的ANCA ,固定中性粒细胞 ELISA可作为血清ANCA检测的重要手段 .
: Objective To establish a fixed neutrophil ELISA to analyze the diagnostic value of serum ANCA in UC.Methods Serum of 25 ulcerative colitis and 26 bacterial colitis serum were detected by ELISA in fixed neutrophils, Type diarrhea serum and 50 normal control serum ANCA, the serum absorbance value of A were statistically analyzed.Results There was a significant difference (P <0.05) between ANCA in UC group and ANCA in other control groups .A value of AUC in the UC group was 80% higher than that of the normal control group A, with a diagnostic specificity of 89% .Conclusion There are diagnostic value of ANCA in UC serum and ELISA can be used as an important measure of serum ANCA detection .