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探讨多项指标联合检测对诊断恶性腹水的价值。方法 用生化法测定腹水中腺苷脱氨酶(ADA)及总蛋白定量(Tg),用双抗体夹心法间接ELISA检测可溶性白介素-2受体(sIL-2R),用夹心法ELISA检测白介素-8(IL-8)。结果 用本文检测方法,当腹水中sIL-2R>780u/ml、IL-8<40ng/L时,可认为是诊断恶性腹水的两项阳性结果,以上两项指标中任一项阳性时,有可能是恶性腹水,两项均阳性时可诊断为恶性腹水,两项均阴性时可排除恶性腹水。结论 多项指标联合检测可提高诊断恶性腹水的准确性。
To explore the value of combined detection of multiple indicators in the diagnosis of malignant ascites. Methods The adenosine deaminase (ADA) and total protein (Tg) in ascites were determined by biochemical method. Soluble interleukin - 2 receptor (sIL - 2R) was detected by double antibody sandwich ELISA. The interleukin - 8 (IL-8). Results With the detection method of this article, when asIL-2R> 780u / ml in ascites and IL-8 <40ng / L, it can be considered as two positive results in the diagnosis of malignant ascites. When any of the above two indicators is positive, May be malignant ascites, both were positive can be diagnosed as malignant ascites, two negative can rule out malignant ascites. Conclusion Combined detection of multiple indicators can improve the accuracy of diagnosis of malignant ascites.