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槐耳浸膏(Extractum trametes robiniophila murr,ETRM)是一种真菌的提取物,最近的研究表明,ETRM能有效地改善阿霉素(ADR)诱导的肾病大鼠足细胞足突的清除.在本研究中,我们探讨了槐耳浸膏对足细胞骨架重排及足细胞固有分子α-actinin-4、nephrin的调控特点.首先进行分组:槐耳浸膏组:槐耳浸膏(10 mg/mL)+阿霉素(0,5 μmol/L);地塞米松组:地塞米松(1μmol/L)+阿霉素(0.5μmol/L);阿霉素组:阿霉素(0.5 μmol/L);正常足细胞组.采用invitrogen(R)鬼笔环肽直接荧光标记各组足细胞F-actin后,在激光共聚焦显微镜下观察细胞骨架重排情况.Real-time PCR和Western blott分析各组足细胞α-actinin-4、nephrin的表达变化.未经药物预处理的足细胞在阿霉素干预后,骨架发生显著重排.槐耳浸膏预处理的足细胞显示出与地塞米松预处理组相似的稳定足细胞骨架,减轻重排发生的能力.Real-time PCR和Westem blott均提示与正常足细胞相比,槐耳浸膏预处理后足细胞α-actinin-4表达量显著低于阿霉素组,与正常足细胞组无显著差异.但槐耳浸膏预处理不能缓解阿霉素引起的nephrin表达改变.地塞米松预处理组α-actinin-4和nephrin表达水平均显著低于阿霉素组.槐耳浸膏能够减轻阿霉素诱导的足细胞骨架重排,其作用机制可能与调控α-actinin-4的表达有关.“,”The Chinese medicine Extractum trametes robiniophila murr (ETRM) is the extract of a type of fungus.Recent studies have suggested that ETRM efficiently improves the effacement ofpodocyte foot processes in adriamycin (ADR)-induced nephrotic rats.In the present study,we aimed to assess whether ETRM modulated the actin rearrangements of podocytes and involved signaling molecules,including α-actinin-4 and nephrin.Podocytes were treated with ADR (0.5 μmol/L),ADR (0.5 μmol/L) + dexamethasone (Dex) (1 μmol/L),ADR (0.5 μmol/L) + ETRM (10 mg/mL).The F-actin in the podocytes was stained by fluorescent phallotoxins and observed by confocal microscopy.The expression levels of α-actinin-4 and nephrin were tested by real-time PCR and Western blotting analysis.The administration of ETRM could significantly prevent ADR-treated podocytes from actin rearrangement.Both ETRM and Dex could stabilize podocyte actin cytoskeletons.Moreover,α-actinin-4 might act as a potential target for ETRM functionality in podocyte actin rearrangements.However,pretreatment with ETRM could not inhibit the up-regulation of nephrin as a result of ADR treatment.ETRM could improve the cytoskeleton stability in ADR-induced actin rearrangement ofpodocytes via regulating α-actinin-4 expression.