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目的:建立鹰嘴豆芽素A固体脂质纳米粒(BCA-SLN)和纳米脂质载体(BCA-NLC)的包封率和载药量的高效液相色谱测定方法。比较2种纳米粒包封率和载药量的差异。方法:采用超滤离心法分离游离药物,高效液相色谱法测定药物含量。Cosmosil C18色谱柱(4.6 mm×250 mm,5μm);柱温30℃;流动相乙腈-0.1%磷酸(60∶40);流速1 mL.min-1;检测波长260 nm。结果:BCA在0.005～5.000 mg.L-1线性良好(r=1.000)。所制BCA-SLN包封率为(97.15±0.28)%,载药量为(6.38±0.043)%;BCA-NLC包封率为(99.57±0.11)%,载药量为(6.91±0.044)%。比较2种纳米粒包封率和载药量表明均存在显著性差异(P≤0.05)。结论:采用高效液相色谱法结合超滤离心法测定BCA 2种纳米粒包封率和载药量方法准确、高效,NLC具有更高的包封率和载药量。
OBJECTIVE: To establish an HPLC method for determination of entrapment efficiency and drug loading of biochancapsulated BCA-SLN and BCA-NLC. Differences in entrapment efficiency and drug loading between the two nanoparticles were compared. Methods: The free drug was separated by ultrafiltration and the drug content was determined by high performance liquid chromatography. Cosmosil C18 column (4.6 mm × 250 mm, 5 μm); column temperature 30 ℃; mobile phase acetonitrile -0.1% phosphoric acid (60:40); flow rate 1 mL.min-1; detection wavelength 260 nm. Results: BCA was linear at 0.005-5.000 mg.L-1 (r = 1.000). The encapsulation efficiency of BCA-SLN was (97.15 ± 0.28)% and the drug loading was (6.38 ± 0.043)%, and the encapsulation efficiency of BCA-NLC was (99.57 ± 0.11)% and 6.91 ± 0.044 %. Comparing the encapsulation efficiency and drug loading of the two kinds of nanoparticles showed that there were significant differences (P≤0.05). Conclusion: The method of high performance liquid chromatography coupled with ultrafiltration centrifugation to determine the encapsulation efficiency and drug loading of the two kinds of BCA nanoparticles is accurate and efficient. NLC has higher entrapment efficiency and drug loading.