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目的建立滴金免疫法检测流行性乙型脑炎患者血清特异性IgM的方法。方法将乙脑病毒的特异性抗原点于硝酸纤维素薄膜上，用来捕获血清和脑普液标本中的特异性IgM抗体，通过胶体金标记的抗人μ链单克隆抗体结合物来直接显色，阳性者出现红色斑点。结果用本方法与2一流基乙醇耐性试验对比检测血清标本50份和脑脊液标本45份，两法的总符合率分别为98.0％和95.6％。结论本方法简便、快速，适于向基层推广，对乙脑的早期诊断有重要的临床意义。
Objective To establish a method of dropping gold immunization to detect serum specific IgM in patients with Japanese encephalitis. Methods The specific antigen of Japanese encephalitis virus was spotted on nitrocellulose membrane to capture specific IgM antibodies in serum and cerebrospinal fluid specimens and directly by colloidal gold labeled anti-human μ-chain monoclonal antibody conjugates Color, positive red spots appear. Results Compared with 2 first-class ethanol tolerance test, 50 samples of serum and 45 samples of cerebrospinal fluid were detected by this method. The total coincidence rates of the two methods were 98.0% and 95.6% respectively. Conclusion The method is simple, rapid, suitable for promotion to the grassroots and has important clinical significance for the early diagnosis of JE.