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为探讨双孢蘑菇子实体发育不同阶段的蛋白质表达变化,对双孢蘑菇As2796菌株子实体原基期、幼菇期、采收期、开伞期的蛋白质组进行了二维液相色谱串联质谱(i TRAQ-MS/MS)分析,共获得不同肽段5 869个,鉴定到1 059个蛋白质,其中1 007个具有相对定量信息。与双孢蘑菇原基期相比,幼菇期、采收期和开伞期分别有差异蛋白质242、200、240个,分别占鉴定蛋白质总数的24.0%,19.9%和23.8%。对这些蛋白质及不同阶段之间的差异蛋白质进行了系列生物信息学分析,对8个上、下调表达具有连续性的差异蛋白质相关基因进行了荧光定量PCR验证,其中3个蛋白质(错配碱基识别蛋白、细胞色素C1及某推定的未知蛋白质)基因的转录与蛋白质的表达具有较为一致的趋势。这些结果为后续双孢蘑菇子实体发育相关基因的功能研究奠定了良好的基础。
In order to explore the protein expression changes in different stages of Agaricus bisporus fruiting body development, two-dimensional liquid chromatography tandem mass spectrometry (MS / MS) was carried out on the proteomes of the fruiting body of Agaricus bisporus As2796 in the primordial stage, young mushroom stage, harvest stage, i TRAQ-MS / MS). A total of 5 869 different peptide fragments were obtained, of which 1 059 proteins were identified, of which 1 007 had relative quantitative information. Compared with the original stage of Agaricus bisporus, there were 242,200,240 differential proteins in young mushroom stage, harvest stage and open-draw stage, accounting for 24.0%, 19.9% and 23.8% of the total identified proteins, respectively. A series of bioinformatics analyzes were performed on these proteins and differential proteins at different stages. Eight differential proteins related to the up-and down-regulated expression were verified by real-time PCR. Three proteins (mismatched bases Recognition protein, cytochrome Cl and a putative unknown protein) gene transcription and protein expression has a more consistent trend. These results laid a good foundation for the functional study of the subsequent developmental genes related to fruiting bodies of Agaricus bisporus.