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以超表达平邑甜茶(Malus hupehensis Rehd.var.pingyiensis Jiang)SnRK1(蔗糖非发酵–1–型相关蛋白激酶–1)基因MhSnRK1的番茄株系O-7及野生型番茄(Solanum lycopersicum)为试材,研究MhSnRK1对番茄植株碳代谢的影响。结果表明,与野生型番茄相比,超表达MhSnRK1番茄数字基因表达谱分析显示,光合途径中差异表达的10个基因中7个基因转录被上调;功能叶片的净光合速率日变化平均值提高20.3%;嫩叶及功能叶SnRK1酶活性比野生型功能叶提高20.6%和25.0%,可溶性糖提高57.7%和27.0%;超表达MhSnRK1番茄嫩叶SnRK1酶活性明显高于功能叶,淀粉含量提高了近1倍,而野生型番茄嫩叶及功能叶差异不明显。海藻糖处理后,超表达MhSnRK1番茄和野生型番茄净光合速率都下降,且野生型下降幅度大于超表达MhSnRK1番茄;超表达MhSnRK1及野生型番茄嫩叶和功能叶SnRK1酶活性都降低,但超表达Mh Sn RK1番茄仍高于野生型。
Tomato lines O-7 and Solanum lycopersicum which over-expressed MhSnRK1 of Malus hupehensis Rehd. Var. Pingyiensis Jiang SnRK1 (sucrose non-fermentative 1-associated protein kinase-1) Effects of MhSnRK1 on Carbon Metabolism in Tomato Plants. The results showed that compared with wild type tomato, the gene expression profile of MhSnRK1 overexpressed tomato showed that seven of the ten differentially expressed genes in photosynthetic pathway were up-regulated; the average daily change of net photosynthetic rate of functional leaves was increased by 20.3 %. The activities of SnRK1 in young leaves and functional leaves increased by 20.6% and 25.0% and soluble sugar increased by 57.7% and 27.0%, respectively. The activity of SnRK1 in young leaves with overexpression of MhSnRK1 was significantly higher than that in functional leaves and the content of starch was increased Nearly 1 times, while wild-type tomato leaves and functional leaves was not significantly different. After trehalose treatment, the net photosynthetic rate of over-expressed MhSnRK1 tomato and wild-type tomato decreased, and the wild-type decreased more than that of MhSnRK1 overexpressing MhSnRK1. The activities of SnRK1 in overexpression MhSnRK1 and wild-type tomato decreased, The Mh Sn RK1 expressing tomato was still higher than the wild type.