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目的:观察活血化瘀药对脑出血大鼠脑组织含水量及AQP4表达的影响,以期从分子水平探讨其治疗出血性脑水肿的机制。方法:将80只雄性SD大鼠随机分为假手术组,模型组,活血高、低剂量组(10.4,2.6 g.kg-1),采用自体不凝血注入尾状核法制作大鼠脑出血模型,组织干湿重法计算脑含水量,免疫组织化学法和real-timePCR法检测脑组织AQP4蛋白和基因的表达。结果:模型组大鼠各时间点病灶侧脑含水量、血肿周围组织AQP4蛋白和基因的表达均明显高于假手术组(P<0.01);活血高、低剂量组大鼠病灶侧脑含水量及血肿周围组织AQP4的表达与同一时间点模型组相比均明显降低,活血高、低剂量组间也有统计学意义。结论:活血化瘀药能够减轻脑出血后脑水肿,其机制可能与降低AQP4表达有关。
Objective: To observe the effect of Chinese herbal for promoting blood circulation to remove blood stasis on the brain water content and the expression of AQP4 in rats with intracerebral hemorrhage (ICH), and to explore its mechanism of treating hemorrhagic brain edema at the molecular level. Methods: Eighty male Sprague-Dawley rats were randomly divided into sham-operation group, model group, high-dose and low-dose groups (10.4 and 2.6 g.kg-1) The brain wet weight was used to calculate the brain water content. The expression of AQP4 protein and gene in brain tissue was detected by immunohistochemistry and real-time PCR. Results: At each time point, the expression of AQP4 protein and gene in the perihematomal brain tissue in the model group was significantly higher than that in the sham operation group (P <0.01). The content of the lateral brain water in the high and low dose groups And the expression of AQP4 in the perihematoma tissue were significantly lower than those in the model group at the same time point. There was also a significant difference between the high and low dose groups. Conclusion: Activating blood circulation and removing blood stasis medicine can relieve cerebral edema after intracerebral hemorrhage. Its mechanism may be related to the decrease of AQP4 expression.