紫花苜蓿根腐病性病害生防放线菌的分离与筛选(英文)

• 作 者: 王兰英 徐林波 何勇 乌兰巴特尔
• 机 构: 海南大学环境与植物保护学院,中国农业科学院草原研究所,青岛海源草坪有限公司,
• 出 处: Plant Diseases and Pests
• 发表日期: 2010年02期
• 关键词: 根腐 拮抗作用 对峙培养法 内蒙古赤峰 紫花苜蓿 土样 害生 拮抗菌株 靶标菌 生物防治

[目的]从内蒙古采集的10个土样中分离筛选出对苜蓿根腐病病原具有很好拮抗作用的放线菌。[方法]利用梯度稀释分离法从来自内蒙古赤峰地区的10个土样中分离得到91株放线菌。以3株苜蓿根腐病菌为靶标菌,采用平皿对峙培养法,对分得菌株进行皿内拮抗作用测定。[结果]获得5株对供试苜蓿根腐病菌有较强抑制作用的拮抗菌株,编号为1-3-6、4-4-2、6-2-25、6-2-27、7-2-13,分别来自1号、4号、6号及7号土样,占分离菌株的5.50%。[结论]该研究可为苜蓿根腐病生物防治奠定一定基础。

[Objective] The actinomycetes isolated from 10 soil samples collected in Inner Mongolia were used to screen antagonistic pathogen of alfalfa root rot. [Method] With the method of gradient dilution, 91 actinomycetes were isolated from 10 soil samples from Chifeng area in Inner. Three strains of alfalfa root rot were used as target bacteria, and the antagonistic effect was determined by plate confrontation culture method. [Result] Five antagonistic strains were obtained, which had strong inhibitory effect on the root rot of Alfalfa, numbered as 1-3-6, -4-4-2, 6-2-25, 6-2-27 and 7- 2-13, respectively, from 1, 4, 6 and 7 soil samples, accounting for 5.50% of isolates. [Conclusion] This research could lay a solid foundation for biological control of root rot of alfalfa.